余氯对小球藻的影响以及损失评估  

Effects of residual chlorine discharged in water on the growth of phytoplankton

作　　者：金施[1,2] 孙岳[3] 徐兆礼[1] 毕亚梅[1] 孙鲁峰[1] 

机构地区：[1]中国水产科学研究院东海水产研究所农业部海洋与河口渔业重点开放实验室,上海200090 [2]上海海洋大学,上海200090 [3]国家海洋局海洋咨询中心,北京100860

出　　处：《生态学报》2014年第19期5425-5433,共9页Acta Ecologica Sinica

基　　金：2012年农业部专项;海洋公益性课题(200905010-12)

摘　　要：滨海电厂和液化天然气(LNG)工程排出的余氯水可能对周边海域浮游植物造成不利的影响,从而成为破坏海洋生态系统平衡的重要因素之一。在实验室条件下研究余氯对浮游植物的抑制作用和造成的损失。余氯抑制试验用小球藻(Chlorella sp.)进行,依据工程余氯排放工况,余氯实验浓度设计为0、0.02、0.05、0.10和0.20 mg/L 5个水平;实验温度为27℃,代表夏季;观察时间分别为0、24、48、72和96h。结果表明:高浓度(0.20mg/L)余氯对小球藻生长有抑制作用,最大值出现在48h时,48h之后抑制作用基本维持在同一水平;低浓度(0.02mg/L)对小球藻有促进作用;0.05mg/L组与0.10mg/L组在实验时间24、48h和72h时表现出余氯对小球藻生长抑制作用,在96h时与对照组藻类生长情况差异不显著。采用积分方程计算累计损失率,参考上海LNG工程2009年9月浮游植物细胞丰度值,计算得出工程余氯排放在72h内所造成的藻类理论损失量为1.97×1012个。The residual chlorine （RC） discharged from coastal power plant may have adverse effects on the phytoplankton in the surrounding waters, affecting the balance of the ecological system. To help us understand the inhibition mechanism of RC on phytoplankton, the RC discharged from the cooling water of a liquefied natural gas （LNG） project was simulated under laboratory conditions. The experimental subject was Chlorella sp. The experimental concentrations of RC were 0, 0. 02, 0.05, 0.10 mg/L and 0.20 mg/L, based on the engineer＇s estimates of RC density in discharges. The simulation was conducted at 27℃, which imitated summer temperature. The testing times were 0, 24, 48, 72 h and 96 h. Results showed that at 27℃, the RC at high concentration （0.20 mg/L） had the greatest inhibitive effect on the growth of Chlorella sp., with the strongest retarding effect （25.95%） after 48h. The inhibition effect on Chlorella sp. by the highest concentration of RC remained constant after 48h. At a low concentration （0.02 mg,/L）, the RC promoted the activity of Chlorella sp., which is in accordance with the hormesis mechanism. At 0.05 mg/L and 0.10 mg/L, the RC displayed an inhibitive effect at 24, 48 h and 72 h, and there were no marked inhibitive difference between these three time points. After 96h, there was no significant difference in the inhibitive effect of these two RC concentrations. Based on the algae growth rhythm, a growth model was fitted, finding a significant linear relationship between the control group （CG） and experimental groups （EG）. The difference equation was calculated by subtracting the growth equation from the CG equation, creating the corresponding integral equation. Finally, the cumulative loss ratio （CLR） was calculated using the relative loss integral equation. At 0. 05mg/1 RC, CLR increased between 0 h and 72 h, with a maximum value was 8.73% at 72 h and 96 h. At 0.10 mg/1 RC, the CLR was the same as at 0.05rag/L, apart from after 96h, when the 0.10mg/L CLR began to d

关 键 词：余氯 浮游植物 抑制作用 损失量 

分 类 号：X173[环境科学与工程—环境科学] X820