应用随机引物法鉴定伪劣羊肉卷中的肉种成分  

Application of Random Primer Method for the Identifi cation of Species Composition in Fake “Sliced Mutton”

作　　者：王昱[1,2] 叶自霞 聂福平[1] 肖进文[1] 杨俊[1] 袁增壮 王国民[1] 李应国[1] 李正国[1] 

机构地区：[1]重庆大学,重庆400044 [2]重庆出入境检验检疫局,重庆400020 [3]西南大学,重庆400716

出　　处：《中国动物检疫》2014年第10期74-78,83,共6页China Animal Health Inspection

摘　　要：为鉴定市场抽检的来源不明的伪劣"羊肉卷"中的肉种成分,采用随机引物法,PCR扩增肉制品DNA,将阳性产物回收、克隆并测序,然后根据序列信息设计特异性的荧光定量引物进行再次鉴定。结果表明,获得了针对伪劣"羊肉卷"的阳性克隆,序列比对表明样品中含有北极狐或火狐肉成分,经特异性的荧光定量PCR确认样品为火狐肉。结果表明,可以应用随机引物PCR鉴定伪劣"羊肉卷"中的肉种成分。To identify species composition of meat and meat products sampled from market,random primer method was used for PCR amplification of meat DNA,the positive products were recovered,cloned and sequenced,and then the specific real time PCR primers were designed according to the sequence information for re-identification. Positive clones were obtained from the sliced mutton,and sequence alignment indicated that the sliced mutton sample contained Alopex lagopus or Vulpes vulpes meat ingredients. The Vulpes vulpes meat ingredient was confirmed in the sliced mutton sample by specific quantitative PCR. The results showed that the random primer PCR could be applied to the identifica-tion of species origin of meat and meat products .

关 键 词：随机引物法 肉制品 物种成分 鉴定 

分 类 号：TS251.7[轻工技术与工程—农产品加工及贮藏工程]