脊髓神经干细胞对小鼠视网膜移植的研究  

STUDY OF TRANSPLANTATION OF SPINAL NEURAL STEM CELLS INTO MICE RETINA

作　　者：孟晋宏[1] 罗娜[1] 鞠躬[1] 

机构地区：[1]第四军医大学全军神经科学研究所,西安710032

出　　处：《解剖学报》2002年第4期342-345,共4页Acta Anatomica Sinica

摘　　要：目的　研究原代培养的脊髓神经干细胞在小鼠视网膜的整合和分化情况。　方法　利用细胞培养和体内移植技术 ,将原代脊髓神经干细胞 (NSC)移植到不同年龄小鼠的视网膜 ,并对移植后细胞的整合及分化情况进行了免疫组织化学分析。　结果　 1 移植的NSC对组织的整合能力随宿主年龄的增加而降低 ;2 移植的NSC在宿主视网膜内可以分化为星形胶质细胞、少突胶质细胞和神经元。　结论　脊髓原代NSC移植到小鼠视网膜后的整合和分化均受内外因素的调控 。Objective To study the integration and differentiation of spinal neural stem cells after transplantation into mice retina. Methods Primary cultured neural stem cells were transplanted into mice retinas,then the integration ratio and the differentiation pattern of the donor cells were estimated with immunohistochemistry method. Results 1.The integration ratio decreased with the age of the host mice.2.The grafts differentiate into both glia and neuronal cells after transplantation.Conclusion The integration and differentiation of the primary cultured spinal NSC were modulated by both endogenous and exdogenous factors,which provided new proofs for the study of the in vivo differentiation of the NSC.

关 键 词：视网膜移植 脊髓 神经干细胞 细胞培养 免疫组织化学 细胞整合 细胞分化 

分 类 号：R779.6[医药卫生—眼科]