基于T-RFLP和因子分析的香蒲根际细菌群落研究  

The research of cattail rhizosphere bacteria community based on T-RFLP and FA

作　　者：马栋山 熊薇[1,2] 张琼琼[1,2] 郭羿宏 赵文吉[1,2] 郭逍宇[1,2] 

机构地区：[1]首都师范大学资源环境与旅游学院,北京100048 [2]北京市城市环境过程与数字模拟重点实验室-省部共建国家重点实验室培育基地,北京100048

出　　处：《中国环境科学》2014年第10期2684-2691,共8页China Environmental Science

基　　金：国家自然科学基金(40901281);北京市教育委员会科技计划面上项目(KM201310028012)

摘　　要：以北京市永定河王平湿地为研究对象,应用末端限制性片段长度多态性(T-RFLP)对王平湿地再生水补水口及其远离补水口的香蒲根际细菌和氨氧化细菌(AOB)的群落结构及多样性变化进行分析,在此基础上结合MiCA3比对、典范对应分析(CCA)和多元统计中因子分析(FA),解析湿地不同空间细菌群落功能特性的变化.结果显示再生水补水口细菌群落各多样性指数均明显低于远离补水口细菌群落,而氨氧化细菌群落则呈现相反趋势.基于传统的T-RFLP片段MiCA3比对、CCA和FA相结合的分析表明:对细菌群落,占再生水补水口细菌群落总丰度55.6%的菌群与150bp代表的鞘氨醇单胞菌(Sphingomonas sp.)具相似净化功能,并与总有机碳生物化学循环过程具密切关系,其次占再生水补水口上游细菌群落总丰度75.5%的菌群与81bp为代表的假单胞菌属(Pseudomonas sp.)和Geitlerinema sp.具相似净化功能,并与氮的生物化学循环过程具密切关系.占再生水补水口下游细菌群落总丰度68.7%的菌群与138bp代表的小单孢菌属(Micromonospora sp.)具相似净化功能,同时与重金属Cu、V、和Ti生物化学循环具密切关系.对氨氧化细菌,266bp受铵态氮影响较大,占再生水补水口氨氧化细菌群落总丰度65.5%的菌群与266bp代表的Nitrosomonas sp.具相似的生境特征,适合在高氨环境中生长;与58bp所代表的Nitrosospira sp.具相似功能特性菌群丰度在远离补水口样点中较前者呈现显著增加趋势,表征该类菌群适合在相对低氨环境中生长.Water reclamation and reuse have been actively promoted in Beijing, but the potential influences of reclaimed water on the structure and function of microbial community were still poorly understood. Therefore, communities of both bacterial and ammonia oxidizing bacterias in cattail rhizosphere between the samples of reclaimed water outfall （RWO） in the Wang ping Wetland of Yong ding River, Beijing and far from the reclaimed water outfall （FRWO） were compared. Terminal Restriction Fragment Length Polymorphism （T-RFLP） was conducted to quantitatively detect the changes of bacterial and ammonia bacterial community structures and diversity. Then, Microbial Community Analysis Ⅲ, Canonical Correspondence Analysis （CCA） and Factor Analysis （FA） were used to investigate the changes of bacterial and ammonia bacterial community functions in different water qualities. The results were as followed： samples were classified according to the disturbance degree of reclaimed water. The bacterial diversity of RWO was significantly lower than that of FRWO, while the ammonia oxidizing bacterial diversity of RWO was higher than that of FRWO. As for bacteria, the function of root-attached phylotypes in RWO samples, which accounted for 55.6% of the bacterial community, was similar to the Sphingomonas sp. by 150-bp T-RFs. This function might be closely related to the total organic carbon in the biological chemical cycle. The major phylotypes from upstream samples had the same function with Pseudomonas sp.and Geitlerinema sp. by 80-bp T-RFs, whose proportion was 75.5%of the bacterial community, and the function was closely related to nitrogen in the biological chemical cycle. The major phylotypes from downstream samples had the same function with Micromonospora sp.by138-bp T-RFs, whose proportion was 68.7% of the bacterial community, and the function was closely related to metals such as Cu, V and Ti in the biochemical cycle. For ammonia oxidizing bacteria, the function of root-attached phylotypes in RWO sample, w

关 键 词：因子分析(FA) 末端限制性片段长度多态性(T-RFLP)技术 再生水 根际细菌 

分 类 号：X172[环境科学与工程—环境科学]