结核分枝杆菌H37Ra菌株ESAT6基因的克隆及序列分析  

Cloning and sequential analysis of ESAT6 gene of Mycobacterium tuberculosis H37Ra

作　　者：王英[1,2] 薛玉芹[1,2] 王雪梅[1,2] 陈勇[2,3] 李江艳[1,2] 李倩[1,2] 唐洁[2,3] 夏惠[1,2] 方强[1,2] 

机构地区：[1]蚌埠医学院病原生物学教研室,安徽蚌埠233030 [2]蚌埠医学院安徽省感染与免疫重点实验室,安徽蚌埠233030 [3]蚌埠医学院免疫学教研室,安徽蚌埠233030

出　　处：《蚌埠医学院学报》2014年第10期1324-1326,共3页Journal of Bengbu Medical College

摘　　要：目的:从结核分枝杆菌H37Ra菌株克隆早期分泌性抗原靶6(ESAT6)基因并进行序列分析。方法:以结核分枝杆菌H37Ra菌株基因组DNA为模版,用PCR方法扩增ESAT6基因,将扩增产物连接到克隆载体pTG19-T中,并用PCR、单双酶切和测序进行鉴定,以BLAST软件进行序列比对分析。结果:从结核分枝杆菌H37Ra株成功克隆了ESAT6基因,测序表明该片段由288 bp组成,与GenBank所报告的H37Rv基因相比同源性为100%。结论:成功克隆了H37Ra株ESAT6编码基因,其序列与H37Rv标准株ESAT6编码基因完全一致。Objective： To clone and sequence the ESAT6 gene from Mycobacterium tuberculosis（ MTB） H37 Ra. Methods： The encoding gene of ESAT6 was amplified from MTB H37 Ra genomic DNA using PCR technique. The amplified PCR product was subcloned into pTG19-T vector. The target gene was identified by PCR,single and double enzyme digestion,sequencing and sequence alignment analysis with BLAST software. Results： The ESAT6 gene was successfully cloned. The sequencing showed the fragment length was 288 bp,which was homology with the MTB H37 Ra gene reported by GenBank. Conclusions： The coding gene of ESAT6 from MTB H37 Ra is successfully cloned.

关 键 词：结核分枝杆菌 H37Ra株 早期分泌性抗原靶6基因 克隆 序列分析 

分 类 号：R378.91[医药卫生—病原生物学]