黄芩苷锌配合物的合成及其对人宫颈癌HeLa细胞抑制作用  

Synthesis of baicalin-zinc complex and its inhibitory effect on human cervical carcinoma He La cells

作　　者：袁瑞娟[1] 王贝贝[1] 赵爽[1] 沈蒙[1] 徐翊文 陈鑫[2] 谭鹏[1] 陶欧[1] 刘永刚[1] 

机构地区：[1]北京中医药大学药学院,北京100102 [2]北京大学药学院

出　　处：《北京中医药大学学报》2014年第9期625-628,共4页Journal of Beijing University of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(No.81102800);北京中医药大学大学生2014年北京共建项目(No.BIGJ1429)

摘　　要：目的合成黄芩苷锌配合物,并研究其对人宫颈癌He La细胞增殖的抑制作用。方法将黄芩苷与醋酸锌反应得到黄芩苷锌配合物,并采用紫外光谱法、红外光谱法、质谱法对配合物进行表征;采用细胞毒性检测CCK-8法检测黄芩苷和黄芩苷锌对He La细胞增殖的体外抑制作用。结果锌离子可能与黄芩苷分子中葡萄糖醛酸上的羧基结合形成1∶2配合物,黄芩苷锌配合物和黄芩苷对He La细胞的半数抑制浓度(IC50)分别为12.03 mg/L和8.69 mg/L。结论黄芪苷和黄芩苷锌配合物均具有体外抗肿瘤活性,但黄芩苷与锌生成配合物后其抗宫颈癌肿瘤活性降低。Objective To synthesize baicalin-zinc complex,and study its inhibitory effect on human cervical carcinoma HeLa cells.Methods Baicalin-zinc complex was synthesized by reaction between baicalin and zinc acetate,and then the complex was characterized by using ultraviolet-visible absorption spectrum,infrared spectrometry and mass-spectrography.The inhibitory effects of baicalin and baicalinzinc complex on the proliferation of HeLa cells in vitro were detected by using CCK-8 method.Results Zinc ion （1 molecule） might combine with carboxyl of glucuronic acid （2 molecule） in baicalin to generate baicalin-zinc complex （1∶2）.The haft inhibitory concentration （IC50） of baicalinzinc complex and that of baicalin on HeLa cells were,respectively,12.03 mg/L and 8.69 mg/L.Conclusion Baicalin and baicalin-zinc complex both have anti-tumor activities in vitro,but when baicalin combines zinc to generate complex,the anti-cervical carcinoma activity will decrease.

关 键 词：黄芩苷 黄芩苷锌配合物 HELA细胞 

分 类 号：R284.3[医药卫生—中药学]