菱花粉生活力测定方法研究  

Research on Methods for Pollen Viability Determinationof Water Chestnut

作　　者：李双梅[1] 柯卫东[1] 彭静[1] 李峰[1] 钟兰[1] 孙亚林[1] 

机构地区：[1]武汉市蔬菜科学研究所,430065

出　　处：《长江蔬菜》2014年第18期45-47,共3页Journal of Changjiang Vegetables

基　　金：武汉市农科院创新项目"菱传粉生物学及杂交技术研究"(Cx201408)

摘　　要：为了探索菱花粉生活力的最佳测定方法,以孝感红菱花粉为试材,利用无机盐培养法、MTT(四甲基噻唑蓝)染色法和TTC(氯化三苯基四氮唑)染色法对花粉生活力进行测定。研究结果表明,培养基中添加低浓度的蔗糖和硼酸对菱花粉的萌发有促进作用,菱花粉萌发的最适培养基为10%蔗糖+0.005%硼酸,萌发率达65.9%;用MTT染色法和TTC染色法测定菱花粉生活力,染色率仅为36.9%和27.1%,效果不理想。In order to explore suitable methods for pollen viability determination of water chestnut, the pollen viability of cultivar Xiaoganhongling was determined by using the methods of inorganic salt culture, MTT staining and TTC staining. The results showed that the medium supplemented with low concentrations of sucrose and boric acid could promote the germination of pollen, and the optimum medium for pollen germination was 10% sucrose＋0.005% boric acid, with the germination rate of 65.9%. The MTT staining and TTC staining methods were not ideal for pollen viability determination of water chestnut, because their dyeing rates were merely 36.9% and 27.1%.

关 键 词：菱 花粉 生活力 萌发率 

分 类 号：S645.4[农业科学—蔬菜学]