自交不亲和杏李品种‘风味玫瑰’授粉早期花柱蛋白质差异分析  

Analysis on Differences of Protein in Stylar Canal During Early Pollination Associated with Self-incompatible Prunus domestica×armeniaca ‘Fengweimeigui'

作　　者：何兴波 雷莉莉[1,2] 杨绍彬[1,2] 傅建敏[1,2] 李芳东[1,2] 

机构地区：[1]国家林业局泡桐研究开发中心,河南郑州450003 [2]中国林业科学研究院经济林研究开发中心,河南郑州450003

出　　处：《林业科学研究》2014年第5期590-597,共8页Forest Research

基　　金：公益项目"第二代杏李种间杂交新品种及栽培技术引进"(No.2014-4-20)

摘　　要：采用双向凝胶电泳和质谱检测技术,对自交不亲和的杏李品种‘风味玫瑰’自花及异花授粉(‘风味玫瑰’ב恐龙蛋’)的花柱进行差异性蛋白质组学研究。研究结果表明,自花授粉与异花授粉有79个差异蛋白点,表达量差异在2倍以上的蛋白点有43个,其中自花授粉中表达丰度明显升高的蛋白点有6个,表达丰度明显降低的蛋白点有37个。只有34个蛋白点在质谱分析中成功鉴定,其中5种蛋白质分别为催化调节蛋白、防御/胁迫蛋白、结构蛋白等。这5种蛋白质直接或间接地影响花粉管在花柱中的生长。This study focused on the differences of protein in stylar canal between that from self-incompatible Prunus domestica × arrneniaca ＇ Fengweimeigui＇ and that from cross-pollination （ACP） （ ＇ Fengweimeigui＇ ×＇ Konglong- dan＇）. Two dimension difference gel electrophoresis （2D-DIGE） and mass spectrum （MS） detection techniques were used in this study. The results showed that there were 79 different protein spots in stylar canal between that from self-pollination and that from cross-pollination, and there was a huge difference, more than double, in terms of protein expression among 43 protein spots, including 6 up-expressed and 37 down-expressed in self-pollination stylar canal compared with that in cross-pollination stylar canal. Only 34 protein spots were identified unambiguously through mass spectrometry analysis, including 5 kinds which were catalytic protein, defence/stress protein, and structural protein, respectively.

关 键 词：'风味玫瑰’ 自交不亲和 花柱 蛋白质组学 质谱鉴定 

分 类 号：S725.7[农业科学—林木遗传育种]