米诺环素对脑缺血再灌注大鼠脑保护作用的实验研究  

An experimental research of Minocycline's protective effect on focal cerebral ischemia / reperfusion in rats

作　　者：石岩[1] 赵颖[1] 付婧[1] 杜叶平[1] 

机构地区：[1]江苏省淮安市第二人民医院急诊科,江苏淮安223002

出　　处：《安徽医药》2014年第12期2240-2243,共4页Anhui Medical and Pharmaceutical Journal

摘　　要：目的探讨米诺环素(MC)的脑缺血保护作用及可能机制。方法健康雄性SD大鼠(250±30)g随机分成假手术组、缺血再灌注组、MC低剂量干预组、MC高剂量干预组,建立大鼠右侧大脑中动脉阻断(middle cerebral artery occulusion,MCAO)局灶性脑缺血再灌注模型,按再灌注不同时点(2、6、12、24和48 h)随机分为5个亚组,每组6只,用于神经功能缺损评分、脑梗死面积测定及反转录聚合酶链反应法(RT-PCR)半定量检测m GluR1-mRNA的表达水平。结果造模术后,缺血再灌注组及MC干预组均出现一定程度神经功能障碍,与缺血再灌注组相比,MC干预组神经功能评分降低,脑梗死面积减少,m GluR1-mRNA的表达降低,差异有统计学意义(P<0.05)。结论米诺环素可降低缺血再灌注大鼠的神经功能评分,缩小缺血再灌注大鼠的脑梗死面积,减少m GluR1-mRNA表达。这可能是米诺环素神经保护作用的机制之一。Objective To explore the neuroprotective effect and mechanism of minocycline on focal cerebral ischemia /reperfusion . Methods Healthy male SD rats （ 250 ±30 ） g were randomized into sham group , ischemia/reperfusion group , minocycline low dose group,minocycline high dose group ,for establishing the acute focal cerebral ischemia/reperfusion models with 30 rats in each group. According to different reperfusion time （2,6,12,24 and 48 h）,each group was randomized into five subgroups （each group n=6）, which were used for nerve function defect evaluation ,cerebral infarction area measurement and detection of mGluR 1-mRNA expression level by reverse transcription polymerase chain reaction method （ RT-PCR） .Results The ischemia reperfusion group and MC interven-tion group both had nerve dysfunction to a certain degree .Neural function score ,cerebral infarction area and mGluR 1-mRNA expression were reduced in the MC intervention group compared with those in the ischemia reperfusion group （P＆lt;0.05）.Conclusions Minocy-cline can reduce neurologic severity scores , cerebral infarction volume and mGluR 1-mRNA expressions in ischemia-reperfusion rats , which may be one of the neuroprotective mechanism of minocycline .

关 键 词：米诺环素 脑缺血再灌注 谷氨酸 代谢型谷氨酸受体 细胞凋亡 

分 类 号：R743.3[医药卫生—神经病学与精神病学]