GC-MS分析炮制对二神丸中挥发性成分的影响  

Influence of processing on volatile constituents in Ershen Pills by GC- MS

作　　者：耿媛媛[1] 胡昌江[1] 潘新[1] 赵玲[1] 熊瑞[1] 

机构地区：[1]成都中医药大学药学院,四川成都610075

出　　处：《中成药》2014年第10期2148-2151,共4页Chinese Traditional Patent Medicine

基　　金：国家自然科学基金资助项目(81274085)

摘　　要：目的 GC-MS分析二神丸中补骨脂和肉豆蔻的炮制对其4种组合挥发性成分的影响.方法 采用水蒸气蒸馏法提取挥发油,气相色谱条件为毛细管色谱柱（HP-5 mS 5％ Phenyl methyl siloxane,30 mm ×0.25 mm ×0.25 μm）,进样口温度为250℃,程序升温,载气为氦气;质谱条件为电子轰击电离（能量为70 eV）,四级杆温度为150℃,离子源温度为230℃.结果 4种组合分别检测出77、109、89和70种化合物,鉴定出了其中78种化合物的结构,占其挥发油总量的97.20％～98.87％.结论 组合Ⅳ（盐补骨脂＋煨肉豆蔻）中补骨脂酚和黄樟醚的量最低,挥发性成分的总量减少,说明补骨脂和肉豆蔻炮制后入药可降低毒性.AIM To explore the influence of processing on volatile constituents of four combinations of Psora- lea and Nutmeg from Ershen Pills by GC-MS. METHODS The essential oil was extracted by steam distillation. The capillary column（ HP-5 mS 5% Phenyl methyl siloxane, 30 mm ×0.25 mm ×0.25 μn） was applied in the gas chromatography analysis. Its temperature of the injection port was 250℃ , programming temperature was used with Helium carrier gas. Mass spectrum employed electron impact ionization （70 eV ）. Its level four rod temperature was 150℃ and the ion source temperature was 230℃. RESULTS Seventy-seven, one hundred and nine, eighty-nine and seventy kinds of constituents were respectively detected from four combinations and among them, seventy-eight kinds of constituents were identified which accounted for 97.20% -98.87% of volatile oils. CON- CLUSION The bakuehiol content and safrol content from group Ⅳ （salted Psoralea and roasted Nutmeg） are the lowest and their volumes of volatiles oil reduced indicates that the processing can reduce toxicity.

关 键 词：二神丸 挥发性成分 GC-MS 炮制 

分 类 号：R283[医药卫生—中药学]