拉曼光谱分析癌变胃黏膜组织中蛋白质改变的研究  

A preliminary study of protein alterations accompanied with malignant transformation of gastric mucosa using Raman spectroscopy

作　　者：陈瑶[1] 代剑华[1] 周学谦[1] 刘云杰[1] 袁月[1] 彭贵勇[1] 

机构地区：[1]第三军医大学西南医院全军消化病研究所,重庆400038

出　　处：《重庆医学》2014年第29期3875-3878,共4页Chongqing medicine

基　　金：国家自然科学基金项目资助(81272580)

摘　　要：目的研究正常和癌变胃黏膜组织中蛋白质的拉曼特征峰的差异及其意义。方法提取12例正常和癌变胃黏膜组织的蛋白质相关特征峰进行对比分析,结合统计学方法,观察癌变胃黏膜组织中蛋白质构型构象、氨基酸组成等改变。结果和正常胃黏膜组织比较,癌变胃黏膜组织中蛋白质相关拉曼特征峰758cm-1、879cm-1、938cm-1、1271cm-1、1660cm-1等发生了不同程度的移位,蛋白质的氨基酸组成及空间构象等出现了改变;结合蛋白质特征峰的相对峰强进行Fisher判别分析,建立判别函数获得了91.7%准确判别率。结论拉曼光谱是研究癌变胃黏膜组织中蛋白质分子的生化改变的新型有效手段,有助于胃癌的基础机制及临床诊疗研究。Objective To study the difference of Raman characteristic peaks of protein in cancer and normal gastric tissues and its significance .Methods Raman peaks of protein from 12 cancer and normal tissues were analyzed .Combined with statistical anal-ysis ,the alteration of configuration ,structure ,amino acids composition of protein from malignant gastric tissues was further ex-plored .Results Compared with the normal gastric tissues ,Raman characteristic peaks like 758 cm -1 ,879 cm-1 ,938 cm-1 ,1271 cm -1 ,1660 cm-1 shifted to higher or lower wave numbers in various degrees ,which indicated that the composition of amino acids , conformation of protein had changed .Meanwhile ,relative peak intensity of amino acids was applied in Fisher discriminate analysis and an accuracy of 91 .7% was acquired from the established discrimination function .Conclusion Raman spectroscopy provides an effective approach in studying molecular biochemical alterations accompanied with malignant transformation of gastric mucosa , which makes a contribution to fundamental mechanism research ,clinical diagnosis as well as treatment of gastric cancer .

关 键 词：拉曼光谱 胃肿瘤 蛋白质类 

分 类 号：R735.2[医药卫生—肿瘤]