猪圆环病毒2型广东分离株ORF2基因的克隆和序列分析  

Cloning and Sequence Analysis of ORF2 Gene of Porcine Circovirus Type 2 Isolated from Guangdong Province

作　　者：沈海燕[1,2] 刘志成[1,2] 郭慧霞[3] 周恒[3] 朱余军[3] 张春红[1,2] 

机构地区：[1]广东省农业科学院动物卫生研究所,广东广州510640 [2]广东省兽医公共卫生公共实验室,广东广州510640 [3]华南农业大学兽医学院,广东广州510642

出　　处：《中国畜牧兽医》2014年第10期12-16,共5页China Animal Husbandry & Veterinary Medicine

基　　金：广东省科技计划项目(2012A020602-094;2011B0507-00007);国家自然科学基金(青年科学基金)(3130-2101)

摘　　要：为了解广东部分地区猪圆环病毒2型（porcine circovirus type 2,PCV2）流行毒株的基因型和遗传变异情况,本研究根据GenBank中PCV2的核苷酸序列设计针对ORF2基因的1对特异性引物,从分离于广东省的8株PCV2分离株BL、HD、Li、GD01、GD02、HD01、HD02和HD03株的细胞培养物中扩增ORF2基因,扩增片段克隆到pMD18-T上,获得重组质粒,并对其进行测序。序列分析结果表明,8株PCV2分离株ORF2基因与其他PCV2 ORF2基因核苷酸序列同源性为89.7%~100%,氨基酸序列同源性为87.2%~99.6%,进化分析结果表明,其中7株分离株处于同一分支,属于PCV2b-1A/1B,HD01株属于PCV2b-1C。In order to clarify genotype and genetic variation of porcine circovirus type 2（PCV2）prevalent in some regions of Guangdong province,one pair of specific primers was designed according to the published ORF2 gene sequence of PCV2,and ORF2 genes of PCV2 strain BL,HD,Li,GD01,GD02,HD01,HD02 and HD03isolated from Guangdong province were amplified.The PCR products were cloned into pMD18-T vector and the recombinant plasmids were obtained and sequenced.The results indicated that the ORF2 gene of PCV2 Guangdong isolates had homologies ranging from 89.7%to 100%at nucleotides level and 87.2%to 99.6% at amino acids level with other PCV2 isolates in GenBank.Phylogenetic analyses revealed that 7stains of isolates were clustered into genetic subgroup PCV2b-1A/1B,isolate HD01 was clustered into genetic subgroup PCV2b-1C.

关 键 词：猪圆环病毒2型 ORF2基因 序列分析 

分 类 号：S852.5[农业科学—基础兽医学]