miR-421在胃癌中的表达及对BGC-823细胞增殖的作用  

Expression of miR-421 in gastric cancer and its effect on proliferation of BGC-823

作　　者：吴建红[1] 浦雄勇[1] 张弦[1] 孙王伟[1] 王泽友[2] 王建军[1,3] 

机构地区：[1]江苏大学附属昆山医院检验科,江苏昆山215300 [2]中南大学肿瘤研究所,湖南长沙410078 [3]中南大学湘雅医院检验科,湖南长沙410078

出　　处：《实用肿瘤杂志》2014年第5期432-435,共4页Journal of Practical Oncology

基　　金：昆山市科技计划项目(KS1347)

摘　　要：目的探讨miR-421在胃癌细胞中的表达水平及对BGC-823胃癌细胞增殖的作用。方法利用PCR方法分析miR-421在胃癌组织细胞及多种胃癌细胞株中的表达水平,并通过RNA转染技术将miR-421 mimics与miR-421 inhibitors转染BGC-823胃癌细胞;通过CKK-8试剂盒检测转染后BGC-823细胞的增殖情况。结果 miR-421在胃癌组织细胞及多种胃癌细胞株中均表达增强(P<0.05);miR-421 mimics转染BGC-823后,癌细胞增殖能力增强(P<0.05),而miR-421 inhibitors转染的BGC-823细胞则增殖力受到明显抑制(P<0.05)。结论 miR-421在胃癌组织细胞及多种胃癌细胞株中均过表达,而miR-421可能直接或间接参与调控BGC-823胃癌细胞的增殖。Objective To investigate the expression of miR-421 in gastric cancer and its effect on cancer cell proliferation. Methods The expression level of miR-421 in gastric cancer tissues and gastric carcinoma cell lines were analyzed by PCR. miR-421 mimics or miR-421 inhibitors were transfected into gastric carcinoma BGC-823 cells. Cell proliferation in transfected BGC-823 cells was detected by CKK-8 kit. Results miR-421 was over-expressed in human gastric cancer cells and several gastric carcinoma cell lines（ P 0. 05）. The growth of BGC-823 cells was promoted significantly after transfected with miR-421 mimics（ P〈 0. 05）,while the proliferation was inhibited after transfected with miR-421 inhibitors（ P〈 0. 05）. Conclusion miR-421 is over-expressed in gastric carcinoma cells and miR-421 may promote the proliferation of gastric carcinoma BGC-823 cells.

关 键 词：胃肿瘤 微RNAS 转染 细胞增殖 聚合酶链反应 

分 类 号：R735.2[医药卫生—肿瘤]