重症急性胰腺炎大鼠胃窦肌间血管活性肠肽神经元的变化  

Changes of vasocative intestinal peptide neurons in the myenteric plexus of gastric antrum in rats with severe acute pancreatitis

作　　者：张诚[1] 林中[1] 袁园[1] 段泽辉[1] 

机构地区：[1]桂林医学院附属医院消化内科,桂林医学硕士研究生541001

出　　处：《医学研究生学报》2014年第9期932-935,共4页Journal of Medical Postgraduates

基　　金：广西壮族自治区卫生厅中医药科技专项课题(GZ-KZ10-125)

摘　　要：目的重症急性胰腺炎(severe acute pancreatitis,SAP)时常见胃肠动力的下降,甚至胃肠功能衰竭。文中旨在探讨SAP伴胃肠动力障碍大鼠胃窦肌间神经丛血管活性肠肽(vasoactineintrestinalpeptide,VIP)阳性神经元的变化。方法20只SD大鼠按随机数表法分为假手术组和SAP模型组。SAP模型组采用0.5 g/L牛黄胆酸钠水溶液逆行胰胆管注射的方式制作SAP模型。术后24h进行胰腺组织病理评分、血清淀粉酶、C-反应蛋白(C-reactionprotein,CRP)、胃排空率及胃窦肌间神经丛VIP阳性神经元表达的比较。结果 SAP模型组较假手术组胃排空率降低[(31.49±3.96)%vs(70.99±1.82)%,P<0.05],胰腺组织病理评分、血清淀粉酶、CRP及胃窦肌间神经丛VIP阳性神经元较假手术组表达均升高[(14.63±1.06)分vs(1.60±0.52)分、(2061.25±434.86)U/L vs(360.20±30.29)U/L、(140.10±17.07)μg/mL vs(35.71±6.43)μg/mL、(22.45±4.10)%vs(10.72±1.92)%,P<0.05]。结论 SAP伴胃肠动力障碍大鼠胃窦肌间神经节VIP阳性神经元发生了重塑,VIP阳性神经元的重塑与SAP伴胃肠动力障碍的神经机制有关。Objective Patients with severe acute pancreatitis （ SAP） always suffer decrease in gastrointestinal motility , even gastrointestinal failure.The article aimed to investigate the changes of vasoactive intestinal peptide （VIP） in the myenteric plexus of the gastric antrum in rats with severe acute pancreatitis （ SAP） complicated by gastrointestinal dysmotility . Methods Twenty Sprague Dawley rats were randomly allocated into two groups：control group and SAP group .Acute pancreatitis was induced by intraductal infu-sion of 5%sodium taurodeoxycholate .24 h after operation , records were made on pathological evaluation of pancreatic lesion , amylase, C-reactive protein （ CRP） , gastric emptying rate and the percentage of VIP neurons in the total neurons . Results Compared with the control group, the gastric emptying rate in the SAP group was significantly lower （[31.49 ±3.96]% vs [70.99 ±1.82]%）.The score of pancreatic lesion, amylase, CRP and the percentage of VIP neurons was significantly higher （[14.63 ±1.06]score vs [1.60 ± 0.52]score, [2061.25 ±434.86]U/L vs [360.20 ±30.29]U/L, [140.10 ±17.07]μg/mL vs [35.71 ±6.43]μg/mL, [22.45 ± 4.10]%vs [10.72 ±1.92]%）（P＆lt;0.05）. Conclusion Plas-ticity took place in VIP neurons in the myenteric plexus of gastric an-trum in rats with SAP complicated by gastrointestinal dysmotility , which was connected with neural mechanism underlying SAP compli-cated by gastrointestinal dysmotility .

关 键 词：重症急性胰腺炎 胃肠动力障碍 血管活性肠肽 

分 类 号：R576[医药卫生—消化系统]