短串联重复序列基因座与肺癌发病风险的相关性研究  

Onset risk of lung cancer and short tandem repeat polymorphisms

作　　者：张雷[1] 强勇[1] 刘小龙[1] 黄海嵘[1] 刘灿辉[1] 申翼[1] 

机构地区：[1]南京军区南京总医院心胸外科,南京医学硕士210002

出　　处：《医学研究生学报》2014年第9期945-948,共4页Journal of Medical Postgraduates

基　　金：国家自然科学基金(81172032)

摘　　要：目的肺癌的发生、发展与个体的遗传背景关系密切。文中旨在研究肺癌发病风险与短串联重复序列(short tandem repeat,STR)基因座的相关性,为深入探索肺癌的发生机制提供新的研究途径。方法采用PCR方法结合毛细管电泳对南京及其周边地区120例肺癌患者(病例组)和156例健康个体(对照组)的外周静脉血样进行15个STR基因座的多态性分析,根据2组在15个STR基因座的等位基因频率分布的差异性来推断与肺癌发生相关的易感因素或抗性因素。结果病例组与对照组分别在CSF1PO、D19S433、D3S1358基因座等位基因10、15.2、16之间的分布差异有统计学意义(P<0.05),OR值均>1。病例组与对照组在CSF1PO、D19S433基因座等位基因14、14.2之间的分布差异有统计学意义(P<0.05),OR值均<1。结论 CSF1PO、D19S433、D3S1358基因座等位基因10、15.2、16可能是与肺癌发生相关的易感因素,CSF1PO、D19S433基因座等位基因14、14.2是与肺癌发生相关的抗性因素。Objective The onset and progression of lung cancer correlate closely to individual genetic background .This study evaluated the correlation between the onset risk of lung cancer and short tandem repeat （STR） polymorphisms in order to find a new pathway for investigating the pathogenesis of lung cancer . Methods Using PCR and electrophoresis on 15 STR loci , we studied the gene and genotype frequencies of peripheral vein blood specimens from 120 lung cancer patients and 156 healthy individuals in Nan-jing area.According to the differences in the allele distribution of the 15 STR loci, we evaluated the susceptive and/or resistant factors relevant to lung cancer . Results There were statistically significant differences between the lung cancer patients and healthy controls at alleles 10, 15.2, and 16 of the loci CSF1PO, D19S433, and D3S1358 （P〈0.05, OR〉1）, as well as at alleles 14 and 14.2 of the loci CSF1PO and D19S433 （P〈0.05, OR〈1）. Conclusion It is possible that alleles 10, 15.2 and 16 of the loci CSF1PO, D19S433, and D3S1358 are susceptive factors and alleles 14 and 14.2 of the loci CSF1PO and D19S433 are resistant factors relevant to lung cancer .

关 键 词：肺癌 短串联重复序列 易感因素 抗性因素 

分 类 号：R734.2[医药卫生—肿瘤]