Snail和E-cadherin在人乳腺癌的表达及临床意义  

Expression and clinic significance of Snail and E-cadherin in human breast cancer

作　　者：何莲[1] 刘骞[1] 冯济龙[2] 黄波[1] 

机构地区：[1]辽宁省肿瘤医院病理科,沈阳110042 [2]沈阳市第五人民医院放疗科,沈阳110023

出　　处：《解剖科学进展》2014年第5期404-407,共4页Progress of Anatomical Sciences

基　　金：辽宁省自然科学基金(NO.20102122)

摘　　要：目的探讨Snail和E-cadherin在乳腺癌组织中的表达及其临床意义。方法采用免疫组织化学方法检测199例乳腺癌组织和60例乳腺腺瘤组织中的Snail和E-cadherin蛋白的表达,分析其表达与临床病理参数及预后的相关性,并分析二者表达的相关性。同时采用Real-time PCR法检测20例乳腺癌组织和20例乳腺腺瘤组织中Snail和E-cadherin mRNA的表达,并分析二者表达的相关性。结果 Snail在乳腺癌组织中的表达水平明显高于乳腺腺瘤组织,E-cadherin在乳腺癌组织中的表达水平明显低于乳腺腺瘤组织(<0.05)。Snail的表达与乳腺癌组织学分级、组织学类型、TNM分期及淋巴结转移密切相关(<0.05);E-cadherin的表达与乳腺癌组织学分级和淋巴结转移密切相关(<0.05)。Snail高表达的患者预后明显差于低表达的患者(<0.05);而E-cadherin高表达的患者预后明显优于低表达的患者(<0.05)。乳腺癌组织中Snail和E-cadherin表达具有显著负相关(<0.05)。结论乳腺癌组织中Snail和E-cadherin表达呈负相关,与组织学分级等临床病理参数密切相关。Objective To investigate the expression of Snail and E-cadherin in breast cancer and its clinical significance. Methods The expressions of Snail and E-cadherin in 199 cases of breast cancer and 60 cases of breast fibroadenoma were detected by immunohistochemistry and Real-time PCR respectively, the relationship of Snail and E- cadherin expression with clinicopathological parameters and prognosis was analyzed. Results The expression level of Snail protein and mRNA was significantly higher in breast cancer than in breast fibroadenoma, but lower for E-cadherin in breast cancer than in breast fibroadenoma, with significant negative correlation between Snail and E-cadherin expressions in breast cancer. The expression of Snail was closely related to histological grade, histological type, TNM stages and lymph node metastasis and the expression of E-cadherin was closely related to lymph node metastasis and histological grade. The patients with high expression of Snail had worse prognosis, While patients with low expression of E-cadherin had worse prognosis. Conclusion The expression of Snail and E-cadherin in breast cancer was negatively correlated, and closely related to clinical pathological parameters.

关 键 词：SNAIL E-CADHERIN 人乳腺癌 预后 

分 类 号：R737.9[医药卫生—肿瘤]