人肝癌细胞电阻抗特性的实验研究  

Experimental Study on Electrical Impedance Properties of Human Hepatoma Cells

作　　者：方云[1] 汤治元[1] 张倩[1] 赵鑫[1] 马青[1] 

机构地区：[1]宁波大学医学院,宁波315211

出　　处：《生物医学工程学杂志》2014年第5期1070-1074,共5页Journal of Biomedical Engineering

基　　金：国家自然科学基金资助项目(51277099);宁波市自然科学基金资助项目(2012A610211)

摘　　要：在0.01~100MHz频率内,使用Agilent 4294A阻抗分析仪测量了人肝癌SMMC-7721细胞的交流阻抗,通过电阻抗谱、Bode图、Nyquist图和Nichols图观察了细胞体积分数（CVF）对肝癌细胞电阻抗特性的影响。结果表明：1频率依赖性：电阻抗实部增量、虚部增量（ΔZ′、ΔZ″）、幅模增量（Δ｜Z＊｜）和相位角增量（Δθ）皆随频率发生变化;2CVF依赖性：当CVF增加时,低频极限增量值（ΔZ′0、Δ｜Z＊｜0）、峰值（ΔZ″p、Δθp）、曲线面积和半径（Nyquist图、Nichols图）均随之增大;3存在两个特征频率：第一特征频率（fC1）和第二特征频率（fC2）,分别来源于细胞膜与细胞外液、细胞质交界面的极化作用。结论：电阻抗谱方法能够观察人肝癌细胞电特性,可用于探讨肝癌细胞电生理机制变化,为进一步筛选抗肿瘤药物提供研究手段和观测指标,具有重要的理论价值和潜在的应用前景。The AC impedance of human hepatoma SMMC-7721 cells were measured in our laboratory by Agilent4294 Aimpedance analyzer in the frequency range of 0.01-100 MHz.And then the effect of hematocrit on electrical impedance characteristics of hepatoma cells was observed by electrical impedance spectroscopy,Bode diagram,Nyquist diagram and Nichols diagram.The results showed that firstly,there is a frequency dependence,i.e.,the increment of real part and the imaginary part of complex electrical impedance（ΔZ′,ΔZ″）,the increment of the amplitude modulus of complex electrical impedance（Δ｜Z＊｜）and phase angle（Δθ）were all changed with the increasing frequency.Secondly,it showed cell volume fraction（CVF）dependence,i.e.,the increment of low-frequency limit（ΔZ′0,Δ｜Z＊｜0）,peak（ΔZ″p,Δθp）,area and radius（Nyquist diagram,Nichols diagram）were all increased along with the electric field frequency.Thirdly,there was the presence of two characteristic frequencies：the first characteristic frequency（fC1）and the second characteristic frequency（fC2）,which were originated respectively in the polarization effects of two interfaces that the cell membrane and extracellular fluid,cell membrane and cytoplasm.A conclusion can be drawn that the electrical impedance spectroscopy is able to be used to observe the electrical characteristics of human hepatoma cells,and therefore this method can be used to investigate the electrophysiological mechanisms of liver cancer cells,and provide research tools and observation parameters,and it also has important theoretical value and potential applications for screening anticancer drugs.

关 键 词：人肝癌细胞 电阻抗谱 BODE图 Nyquist图 Nichols图 

分 类 号：R735.7[医药卫生—肿瘤] R318.14[医药卫生—临床医学]