无羁萜-3β-醇抑制自身免疫性脑脊髓炎的作用研究  

Inhibition Effect of Non Custodial Terpenes-3 β-Alcohol to Autoimmune Encephalomyelitis

作　　者：郭喜霞[1] 杨静 黄宁[1] 万仁玲[1] 李昭辉[1] 许高威 尹雅玲[1] 李鹏[1] 

机构地区：[1]新乡医学院邮编,453003 [2]濮阳市卫生学校

出　　处：《天津医药》2014年第10期965-968,I0001,I0002,共6页Tianjin Medical Journal

基　　金：国家自然科学基金资助项目(81200836);河南省科技厅科技攻关项目(132102310247);新乡医学院重点研究领域招标课题(ZD2011-30)

摘　　要：目的研究无羁萜-3β-醇对实验诱导的豚鼠自身免疫性脑脊髓炎(AE)的抑制作用。方法用25、50和100 mg/kg剂量的无羁萜-3β-醇对实验诱导的AE豚鼠进行灌胃8周。分别检测血浆CD4+/CD8+,白细胞介素(IL)-1、2、6、10,血浆神经肽Y(NPY)、β-内啡肽(β-EP),脑组织转化生长因子(TGF)-β、基质金属蛋白酶(MMP)-2、一氧化氮合酶(NOS)及白细胞分化抗原CD3的含量。光镜观察豚鼠大脑神经元形态学变化,电镜观察神经元超微结构变化,免疫荧光观察NOS在神经元的表达。结果无羁萜-3β-醇抑制实验诱导的AE豚鼠CD4+/CD8+的升高,抑制血浆IL-1、2、6、10的含量升高,抑制血浆NPY升高及β-EP降低,抑制大脑TGF-β降低和MMP-2、CD3升高以及NOS在神经元胞质内的表达,并维持神经元的正常形态。结论无羁萜-3β-醇具有抑制AE的作用。Objective To study the inhibition effect of non custodial terpenes -3β- alcohol to experimentally induced autoimmune encephalomyelitis in guinea pigs. Methods Different doses （25 mg/kg, 50 mg/kg and 100 mg/kg） of non custodial terpenes -3β- alcohol were given to the experimentally induced autoimmune encephalomyelitis model of guinea pigs by garage for 8 weeks. Plasma levels of CD4＋/CD8＋, IL-1, IL-2, IL-6, IL-10, neuropeptide Y （NPY）, beta endorphin （β-EP） , transforming growth factor-β（TGF- β）, matrix metalloproteinase （MMP-2）, nitric oxide synthase （NOS） and leukocyte differentiation antigen CD3 were assessed. The brain neuron morphology changes was observed under light microscopy while its ultrastrueture changes was observed under electron microscope. NOS expression in neurons was observed through immunofluoresee technology. Results Non custodialterpenes -3β- alcohol inhibited the increase of plasma CD4＋/CD8＋, IL- l, IL-2, IL-6, IL-10, MMP-2, CD3 and NPY while decrease of plasma β -EP, brain TGF-β. It also increase NOS expression in neuronal cytoplasm and maintained neuron morphology. Conclusion Non custodial terpenes -3β- alcohol inhibited the experimental autoimmune encephalomyelitis in guinea pig.

关 键 词：脑脊髓炎 自身免疫性 实验性 白细胞介素类 受体 细胞因子 神经肽Y 转化生长因子Β 基质金属蛋白酶2 一氧化氮合酶 抗原 CD 神经元 无羁萜-3β-醇 ' 

分 类 号：R744.3[医药卫生—神经病学与精神病学]