膜联蛋白A5在肝细胞肝癌患者外周血及癌组织中的表达  

Annexin A5 expression in peripheral blood and tumor tissues of patients with hepatocellular carcinoma

作　　者：张爱真 刘海潮[2] 王冬雨[3] 李功权[4] 张嘉凯[4] 潘洁[4] 郭文治[4] 

机构地区：[1]河南省许昌市第二人民医院儿科,461000 [2]洛阳市中心医院肝胆外科 [3]郑州大学第一附属医院感染科 [4]郑州大学第一附属医院肝胆外科

出　　处：《中华实验外科杂志》2014年第10期2119-2121,共3页Chinese Journal of Experimental Surgery

摘　　要：目的 探讨膜联蛋白A5（ANXA5）在肝细胞肝癌患者外周血及癌组织中的表达及其与临床病理特征之间的关系.方法 将148例患者分为肝细胞肝癌组及正常对照组,其中肝细胞肝癌组82例,正常对照组66例,包括肝血管瘤患者47例、肝内胆管结石7例、肝挫裂伤12例;收集两组患者的血清及组织标本,用酶联免疫吸附试验（ELISA）法检测两组患者外周血中ANXA5的含量,免疫组织化学法检测组织标本中ANXA5的表达.结果 肝细胞肝癌患者血清中ANXA5的含量为（46.22 ±21.02） μg/L,明显高于正常对照组的（33.13 ±12.44） μg/L（P＜0.05）,肝细胞肝癌组中ANXA5的阳性表达率为68.29％,正常对照组为22.72％（P＜0.05）;甲胎蛋白（AFP）值较高、分化程度较低、肿瘤直径较大、感染乙型肝炎病毒（HBV）以及发生复发转移的肝癌患者癌组织中ANXA5的表达显著升高（P＜0.05）;但患者血清中ANXA5的浓度与AFP浓度之间无明显相关（P＞0.05）.结论 ANXA5的高表达可能与肝细胞肝癌的发生发展密切相关.Objective To investigate the expression of annexin A5 （ANXA5） in the peripheral blood and tumor tissues of patients with hepatocellular carcinoma （HCC） and its relation with the clinicopathologic characteristics of the carcinoma.Methods 148 patients were divided into HCC group （82 cases） and normal control group （66 cases）.In normal control group,there were 47 cases of liver hemangioma,7 cases of hepatolithiasis,and 12 cases of liver injury.The serum from the peripheral blood and tissue samples were collected.Serum concentration of ANXA5,and the expression of ANXA5 in tissues were analyzed by immunohistochemistry.Results ANXA5 level in serum of HCC patients was （46.22 ± 21.02） μg/L,significantly higher than in control group [（33.13 ± 12.44） μg/L] （P 〈 0.05）.The ANXA5 positive expression rate in HCC and control tissues was 68.29% and 22.72% respectively （P 〈0.05）.In the patients with higher alpha fetal protein （AFP） level,larger tumor size,low differentiation degree,hepatitis B virus （HBV） inffection,recurrence and metastasis,the expression of ANXA5 in the HCC tissues was significantly higher （P 〈 0.05）.But there was no obvious correlation between the ANXA5 concentration and AFP concentration in the serum of patients with HCC （r =0.161,P 〉 0.05）.Conclusion The low expression of ANXA5 is closely related to the tumor progression.Detection of the serum concentration of ANXA5 can be used to diagnose HCC and predict the progression of liver cancer.

关 键 词：癌 肝细胞 膜联蛋白A5 免疫组织化学 

分 类 号：R735.7[医药卫生—肿瘤]