树突状细胞-细胞因子诱导的杀伤细胞自体免疫细胞治疗对转移性大肠癌患者血清微小RNA-29a的影响  

Effect of dendritic cells-cytokine-induced killer cell autologous immune cell therapy on serum microRNA-29a of metastatic colorectal cancer patients

作　　者：范钰[1] 卫菲菲 束永前[1] 张恒[2] 方娜[1] 

机构地区：[1]江苏大学附属人民医院肿瘤研究所,镇江212002 [2]江苏大学附属人民医院普外科,镇江212002

出　　处：《中华实验外科杂志》2014年第10期2290-2292,共3页Chinese Journal of Experimental Surgery

基　　金：江苏省“333工程”科研基金资助项目（BRA2012103）;镇江市社会发展基金资助项目（SH2014047、SH2014050）;江苏大学临床科技基金资助项目（JLY2012006）

摘　　要：目的 探讨树突状细胞（DC）-细胞因子诱导的杀伤细胞（CIK）联合化疗对转移性大肠癌患者外周血微小RNA （miRNA）-29a表达的影响.方法 将39例转移性大肠癌患者随机分为两组,DC-CIK组23例采用XELOX方案＋DC-CIK进行治疗,对照组16例采用XELOX方案进行治疗,采用实时荧光定量聚合酶链反应（FQ-PCR）法检测治疗前及3个周期后两组患者miRNA-29a表达的水平,并观察患者的疗效及评分.结果 经3个周期治疗后,DC-CIK组、对照组miRNA-29amRNA相对表达量均下降,分别为0.515±0.216比1.556±0.228;1.116 ±0.186比1.569±0.289,差异有统计学意义（P＜0.05）;与对照组比较,实验组下降更明显（P＜0.05）.处理组有效率（73.9％）明显高于对照组（31.3％,P ＜0.05）.两组Ⅲ＋Ⅳ度白细胞减少率的比较差异有统计学意义（P＜0.05）.Kamofsky评分比较,DC-CIK组提高优于对照组（P＜0.05）.结论 DC-CIK细胞可有效提高大肠癌整体治疗疗效.下调miRNA-29a是其重要机制之一.Objective To explore the effect and mechanism of dendritic cells （DC）-cytokine-induced killer cell （CIK） combined with chemotherapy on microRNA（miRNA）-29a mRNA in peripheral blood of metastic colorectal cancer.Methods Thirty nine patients with metastic colorectal cancer （CRC） were randomly divided into the treatment group （n =23,treated with DC-CIK combined with chemotherapy） and the control group（n =16,treated with chemotherapy）.miRNA-29a mRNA level in peripheral blood of CRC patients was determined after 2 cycles by real-time fluorescent quantitative polymerase chain reaction （FQ-PCR）.The efficacy and Karnofsky of each group were studied.Results The level of miRNA-29a mRNA of two groups was significantly decreased （P 〈 0.05）,as compared with that before chemotherapy.The treatment group reduced the level of miRNA-29a mRNA more effective than the control group （P 〈0.05）.Total efficacy of treatment group （73.9%） was significantly higher than that of control group （31.3%,P 〈0.05）.There was significant difference in rate of hypolekocytosis at Ⅲ＋ Ⅳ degree between two groups （P 〈 0.05）.Treatment group improved better than the control group in the Karnofsky score （P 〈0.05）.Conclusion DC-CIK can enhance the therapeutic effect probably by reducing the level of miRNA-29a mRNA.

关 键 词：树突状细胞 细胞因子诱导的杀伤细胞 大肠癌 微小RNA-29a 

分 类 号：R735.34[医药卫生—肿瘤]