癌患者血浆、支气管肺泡灌洗液中TMS1/ASC基因启动子甲基化状态研究  

Detection of TMS1/ASC gene promoter hypermethylation in plasma and bronchoalveolar lavage fluid of lung cancer patients

作　　者：王雪峰[1] 周晶[2] 付凯[1] 禹亮[1] 张健[1] 姜久仰[1] 

机构地区：[1]哈尔滨医科大学附属第一医院胸外科150001 [2]哈尔滨医科大学附属第二医院

出　　处：《中华实验外科杂志》2014年第10期2305-2307,共3页Chinese Journal of Experimental Surgery

基　　金：黑龙江省教育厅资助项目（11541134）

摘　　要：目的 探讨肺癌患者血浆和支气管肺泡灌液（BALF）中甲基化诱导静止基因TMS1/ASC启动子甲基化状态及其在肺癌诊断中的价值.方法 应用甲基化特异性聚合酶链反应（MSP）方法检测92例肺癌患者和20例肺部良性病变患者血浆和BALF中TMS1/ASC基因启动子甲基化状态.结果 肺癌组血浆和BALF中TMS1/ASC基因启动子甲基化阳性率分别为27.2％和32.6％.肺良性病变组血浆和BALF中均未检测到TMS1/ASC基因启动子甲基化（x2值分别为6.997、8.908,P＜0.05）.TMS1/ASC基因启动子甲基化状态与肺癌患者的性别、病理类型、临床分期和有无淋巴结转移无明显相关.结论 血浆和BALF中TMS1/ASC基因启动子甲基化检测有助于肺癌诊断.Objective To investigate the status and diagnostic value of hypermethylated TMS1/ASC gene promoter in plasma and bronchoalveolar lavage fluid （BALF） of lung cancer patients.Methods Methylation specific polymerease chain reaction （MSP） was used to detect the hypermethylation status of TMS1/ASC gene promoter in plasma and BALF of 92 cases of lung cancer and 20 cases of benign lesions.Results The positive rate of TMS1/ASC gene promoter methylation in lung cancer patients was 27.2% in plasma and 32.6% in BALF respectively,and promoter methylation in TMS1/ASC gene was not found in plasma or BALF in patients with benign lesions （x2 =6.997,8.908 ; P 〈 0.05）.The positive expression of TMS1/ASC gene promoter methylation in lung cancer patients was not correlated with the gender,histopathological type,clinical stages or lymph node metastasis.Conclusion The hypermethylation status of TMS1/ASC gene promoter in plasma and BALF may be used as an indicator for diagnosis of lung cancer.

关 键 词：肺癌 TMS1/ASC基因 支气管肺泡灌洗液 

分 类 号：R734.2[医药卫生—肿瘤]