X射线对弥漫大B细胞淋巴瘤细胞周期和细胞凋亡的影响  

Impact of X-ray radiation on cell cycle and cell apoptosis of diffuse large B-cell lymphoma

作　　者：季红[1] 郭振峰[2] 孙丰源[3] 

机构地区：[1]天津市眼科医院、天津医科大学眼科临床学院、天津市眼科研究所、天津市眼科学与视觉科学重点实验室,300020 [2]天津市北辰医院眼科,300020 [3]天津市第一中心医院眼科

出　　处：《中华实验眼科杂志》2014年第10期902-905,共4页Chinese Journal Of Experimental Ophthalmology

基　　金：天津市自然科学基金项目（09JCYBJC10500）

摘　　要：背景弥漫大B细胞淋巴瘤（DLBCL）恶性程度高，对放射线敏感，但临床上放射疗法方法各异，合理、有效的放射疗法方案就显得尤其重要。目的探讨X射线对DLBCL细胞周期和细胞凋亡的影响，为其临床治疗提供理论依据。方法将人全身DLBCL细胞系进行常规复苏和培养，将细胞悬液接种后分别暴露于2、4、6、8Gy4种不同剂量的X射线下6、12、24、48h，采用流式细胞仪分析其细胞周期和细胞凋亡的变化，未接受X射线照射的细胞作为空白对照组。结果DLBCL细胞复苏后生长活跃，细胞呈圆形或椭圆形，传2～3代细胞生长状态稳定。随着照射剂量的增加和照射时间的延长，G0-G1期细胞比例逐渐降低，总体差异均有统计学意义（F剂量=45．58，P=0．00；F时间=43．11，P=0．00）；而G2-M期细胞比例逐渐增加，差异均有统计学意义（F剂量i=40．77，P=0．00；F时间=91．91，P=0．00）；细胞凋亡指数逐渐升高，差异均有统计学意义（F剂量=87．36，P=0．00；F时间=37．31，P=0．00）。结论X射线照射将DLBCL细胞阻滞在G2-M期并导致细胞凋亡，其作用呈剂量和时间依赖性。Background The malignant degree of the diffuse large B-cell lymphoma （DLBCL） is high. Radiation therapy is sensitive, but the therapeutic schedule can not be unified, reasonable and effective therapeutic schedule is important in clinic treatment of the DLBCL. Objective This study was to access the effect of X-ray on the cell cycle and cell apoptosis of the DLBCL. Methods DLBCL cell line was cuhured and the cell suspension was inoculated to 12-well cultured plate. The cells exposed to X-ray for 6,12,24,48 hours with the irradiation doses of 2,4,6,8 Gy respectively. The cell proportions of different cycles were assayed by flow cytometer, and cell apoptosis index was evaluated and calculated. Non-irradiation cells served as controls. Results The cells grew well with a good cell vitality and round-like shape, and the growth was stable in generation 2 or 3. With the increase of X-ray doses and the lapse of irradiation time, the cell proportions in GO -Gl phase gradually reduced （Fdose = 45.58, P = 0. 00; Ftime =43.11 ,P=0. 00）. However,cell proportions in G2-M phase were gradually increased as the increase of X-ray doses and lapse of irradiation time （Fdose = 40.77 ,P = 0. 00; Ftime = 91.91, P = 0. 00）. In addition, the apoptotic proportion of the cells was significantly elevated with the increase of X-ray dose and irradiation time （ Fdose = 87.36, P=0. 00; Ftime =37. 31 ,P=0. 00）. Conclusions X-ray irradiation retards DLBCL in the G2-M phase and causes cell apoptosis in dose- and time-dependent manner.

关 键 词：弥漫大B细胞淋巴瘤/治疗 细胞培养 放射疗法 X射线 细胞周期 凋亡 

分 类 号：R733.1[医药卫生—肿瘤]