体内压应力刺激下大鼠髁突软骨细胞的分离、体外培养以及差异蛋白质组学观察  

Isolation,cultivation and differential proteomic analysis of chondrocytes from TMJ condylar cartilage of the mechanical stress stimulated rats

作　　者：李煌[1] 吴拓江[2] 张翔宇[3] 马巧玲[1] 华子春[3] 

机构地区：[1]南京大学医学院附属口腔医院口腔正畸科,210008 [2]江苏省口腔医院口腔正畸科 [3]南京大学医药生物技术重点实验室

出　　处：《实用口腔医学杂志》2014年第5期593-597,共5页Journal of Practical Stomatology

基　　金：国家自然科学资金(编号:81070807;81200764;81470712);南京市卫生局科技发展项目(编号:ZKX0818;YKK11038);青年人才工程项目(编号:2012-B1-228)

摘　　要：目的:观察体内压应力刺激下大鼠髁突软骨细胞的生物学活性和蛋白质表达谱。方法:对大鼠进行III类矫形力应力加载2周,观察髁状突大体形态和组织形态学;体外培养实验鼠和对照鼠软骨细胞,计数细胞收获数和细胞存活率,检测总蛋白质组学水平上差异。结果:压力刺激后的大鼠髁突软骨明显变薄,表面无光泽,缺乏弹性,重量降低(P<0.01);力学刺激后髁突软骨细胞收获率和存活率均下降(P<0.01),形态更加狭长;蛋白质组学结果主要为应激蛋白上调,信号转导蛋白活化,细胞骨架蛋白和细胞增殖代谢相关蛋白的下降。结论:经体内力学加载后,体外分离培养的关节软骨细胞形态、生物学活性以及在蛋白质水平均发生了明显的变化。Objective：To study the biological characteristics of condylar chondrocytes isolated from mechanical stress stimulated TMJ condylar cartilage of rats.Methods：The rat models of mechanical stress stimulated condylar cartilage were made by class III orthope-dic force for 14 days.The cartilage from control and experimental rats were observed in gross view.The cell harvest rate and viability were examined,proteomic analysis was performed.Results：After stress stimulation the thickness,the elasticity of condylar cartilage and number of chondrocytes were significantly reduced.The weight of mandibular cartilage was decreased（P〈0.01）.The harvest rate and the viability rate of the cells were decreased（P〈0.01）.The chondrocytes in the experimental group appeared to be elongated. Proteomic analysis showed that stress-related proteins,signal transduction proteins were up-regulated;cytoskeleton proteins,cell prolif-erative proteins were down-regulated.Conclusion：Mechanical stress stimulation of condylar cartilage may result in biological activity and protein changes of the condylar cartilage chondrocytes.

关 键 词：髁突软骨细胞 力学刺激 细胞培养 大鼠 蛋白质组学 

分 类 号：R783.5[医药卫生—口腔医学]