预培养比格犬干细胞的牙本质片复合牙周膜细胞膜片裸鼠体内移植研究  

Transplantation of stem cell-preseeded dentin slices incorporated with periodontal ligament cell sheets from beagle dog into nude mice

作　　者：卢玉旺 刘宏伟[1] 王晓宇[1] 

机构地区：[1]同济大学口腔医学院牙周科、上海口腔生物医学及转化医学实验室,200072

出　　处：《实用口腔医学杂志》2014年第5期598-602,共5页Journal of Practical Stomatology

基　　金：国家自然科学基金(编号:81271152)

摘　　要：目的:探索预培养干细胞的牙本质片复合牙周膜细胞(PDLCs)膜片对牙周组织再生的可行性。方法:将比格犬牙根制成牙本质片,其上接种骨髓基质干细胞(BMSCs)或PDLCs,应用成骨诱导液或α-MEM培养液培养,电镜观察牙本质片表面细胞附着与基质形成情况。并构建细胞/牙片/膜片/煅烧陶瓷牛骨CBB复合体,裸鼠体内皮下移植,8周后取材HE染色观察。结果:预培养干细胞的牙本质片,电镜检测到表面有足够的细胞和细胞外基质分布。裸鼠体内结果显示牙本质片接种BMSCs或PDLCs经成骨诱导组可见类牙骨质基质形成,但无类牙周膜纤维,未经成骨诱导组无类牙骨质基质形成,但有明显的类牙周膜纤维样组织形成;空白组无类牙骨质基质及类牙周膜纤维样组织形成。结论:在牙本质片上预培养BMSCs或PDLCs,复合PDLC膜片和CBB后异位移植,有利于类牙周膜纤维的形成。加入成骨诱导液诱导,有利于基质的形成。Objective：To observe the effects of dentin proseeded with BMSCs or PDLCs incorporated with PDLCs sheets and calci-nated ceramic bovine bone（CBB）in the reconstruction of periodontal tissue.Methods：Root dentin slices were prepared and pre-seeded with BMSCs or PDLCs from beagle dog.After cultivated in osteoinduction medium（OST groups）orα-modified eagle＆#39;s medi-um（MEM groups）for 3 weeks,the dentin slices observed by scanning electron microscope （SEM）.Then the slices were wrapped successively with PDLCs sheets and CBB as new constructs（BMSCs or PDLCs/dentin/PDLCs sheets/CBB）.The new constructs were transplanted into nude mice subcutaneously,8 week after transplantation,samples were havested and examined by HE staining.Re-sults：Enough cells and extracellular matrices were detected on the dentin slices by SEM in vitro.A little new immature cementum-like tissue without periodontal-like tissue on the surface of the new construcs was observed in OST groups.By contrast,in MEM groups,periodontal-like without immature cementum-like tissue formation was observed.Conclusion：Dentin proseeded with BMSCs or PDLCs incorporated with PDLCs sheets and CBB cultured by MEM can promote periodontal-like tissue regeneration.Cultured with osteooinduction medium can promote cementum-like tissue regeneration.

关 键 词：牙周膜细胞 骨髓基质细胞 细胞膜片 牙周再生 细胞外基质 

分 类 号：R781[医药卫生—口腔医学]