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作 者:李枝桦[1] 王超[1] 王邵坤 杨应明[1] 李觅[1] 朱海滨[1]
机构地区:[1]红云红河烟草(集团)有限责任公司原料部,昆明650201
出 处:《分子植物育种》2014年第5期982-991,共10页Molecular Plant Breeding
基 金:云南中烟工业公司科技项目专项(2010YL04)资助
摘 要:植物间存在着化感作用,适当的种植群落结构可以改善作物的品质。本研究根据烟草与不同香原料植物间作后致香物质的变化情况,探索一种丰富烟叶香气,提高烟叶质量的新途径。烟草与香原料植物(薰衣草,迷迭香,香叶天竺葵,猫薄荷和玫瑰花)间作后,用固相微萃取(SPME)及气相色谱-质谱联用(GC/MS)分析对其叶片中致香物质的变化,在烤烟与香原料植物间栽的小环境动态生态系统中,烟草和香原料植物的致香成分都有变化。与对照烟叶相比,烤烟致香物质的变化呈现了3种不同情况:香原料植物特有的致香成分转移至处理烟叶;香原料植物、对照、处理烟叶共有致香物质含量出现变化;间栽后烤烟出现新的致香成分。进一步研究烟草和香原料植物间相互作用情况,寻找到植物间化感作用影响烟叶致香成分变化的规律,可为丰富烟叶致香成分,构建良性烟田生态系统,建立以烟为主、香原料为辅的耕作制度提供理论支撑,对探索生产绿色、安全的烤烟具重要意义。Agronomic measures mainly affected physiological and biochemical metabolism of tobacco growth and development, and fatherly affected the content and proportion of aromatic matters. In order to explore the intercropping application in the tobacco field, we contrasted the effect of monoculture of tobacco with intercropping effect of K326 and four aromatic plants of Lavandula pedunculata, Rosmarinus officinalis, Pelargonium roseum, Nepeta cataria, Rosa rugosa respectively. Gas Chromatography-Mass Spectra/Olfactory (GC-MS/O) was applied in this dissertation. GC-MS/O is a powerful way in screening aroma compounds in tobacco leaves effectively, which was combined GC-MS to Olfactory. The result showed that the change of flue-cured tobacco aroma substances presented three different situations in the small dynamic ecosystem environment that tobacco intercropped with different fragrant plants: some specific aroma components of fragrant plants transferred to experimental group; the proportion of common aroma substances from fragrant plant, control group and experimental group have changed; new aroma components appeared after intercropping. The aroma components in tobacco can be enriched by intercropping with different fragrant plant.
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