普罗布考对动脉粥样硬化兔主动脉白三烯C_4的影响  

Effect of Probucol on Leukotriene C4in Aorta of Atherosclerotic Rabbits

作　　者：吴乐[1] 黎红华[1] 武强[1] 刘子建[2] 

机构地区：[1]广州军区武汉总医院神经内科,武汉430070 [2]华中科技大学同济医学院解剖学系,武汉430030

出　　处：《神经损伤与功能重建》2014年第5期379-381,共3页Neural Injury and Functional Reconstruction

摘　　要：目的:观察普罗布考对高同型半胱氨酸(Hcy)血症诱导动脉粥样硬化(AS)兔主动脉白三烯C4(LTC4)的影响。方法:将24只雄性新西兰大耳白兔随机分成3组各8只:对照组给予基础饲料喂养;模型组和普罗布考组在基础饲料中加入1%的蛋氨酸;普罗布考组从第12周起用普罗布考(1 g/d)灌胃;饮食量200 g/d,均喂养20周。第20周末用RT-PCR检测动脉血管中白三烯C4合成酶(LTC4S)的mRNA表达,用ELISA检测动脉血管中LTC4的含量。结果:模型组腹主动脉LTC4S的mRNA表达及LTC4的含量较对照组增高(P<0.05),普罗布考组腹主动脉LTC4S的mRNA表达水平及LTC4含量低于模型组(P<0.05)。结论:普罗布考能抑制主动脉LTC4S的mRNA表达,降低主动脉LTC4的含量,该作用可能为其抗AS的机制之一。Objective：To investigate the effect of Probucol on the expression of leukotriene C 4 （LTC4） in the aorta of atherosclerotic rabbits induced by hyperhomocysteinemia. Methods： Twenty-four male rabbits were randomly divided into three groups. The control group was fed on common food, the model group was fed on high-methion-ine food containing 1%methionine, and the Probucol group received high-methionine food and Probucol 1 g/d per-fused into stomach from the 12th week.The food was 200g/d for 20 weeks. The aorta was removed to investigate the leukotriene C4 synthase （LTC4S） mRNA expression by RT-PCR and the protein level of LTC4 by ELISA at 20th week. Results：Compared with those in the control group, the mRNA expression of LTC 4S and the level of LTC4 were increased in the model group （ ＆lt;0.05）. And compared with those in the model group, the mRNA expression of LTC4S and the level of LTC4 were down-regulated in the Probucol group （ ＆lt;0.05）. Conclusion：Probucol could reverse the aortic lesion induced by hyperhomocysteinemia which may be mediated by the suppressive effect on LTC4S mRNA expression and the level of LTC4.

关 键 词：普罗布考 同型半胱氨酸 动脉粥样硬化 白三烯C4 白三烯C4合成酶 

分 类 号：R741[医药卫生—神经病学与精神病学] R741.05[医药卫生—临床医学]