唾液腺腺样囊性癌中RASSF1A表达及与启动子甲基化之间的关系  

The relationship between RASSF1A expression and promoter methylation in salivary adenoid cystic carcinoma

作　　者：李蕾[1] 夏荣辉[1] 张春叶[1] 李江[1] 

机构地区：[1]上海交通大学医学院附属第九人民医院·口腔医学院口腔病理科,上海市口腔医学重点实验室,上海200011

出　　处：《中国口腔颌面外科杂志》2014年第5期407-411,共5页China Journal of Oral and Maxillofacial Surgery

基　　金：国家自然科学基金(81302360);上海市卫生局青年科研项目(20134Y057);教育部博士点基金(20120073110085)~~

摘　　要：目的:研究唾液腺腺样囊性癌(adenoid cystic carcinoma,ACC)中RASSF1A表达及其与启动子区甲基化之间的关系。方法:收集167例原发性唾液腺ACC,亚硫酸盐测序聚合酶链反应(bisulfite sequencing polymerase chain reaction,BSP)和甲基化特异性聚合酶链反应(methylation-specific polymerase chain reaction,MSP)方法检测RASSF1A基因启动子区甲基化状况,免疫组织化学方法检测RASSF1A蛋白表达情况。去甲基化药物decitabine处理ACC细胞系SACC-83后,检测处理前、后RASSF1A基因甲基化及表达情况。应用SPSS18.0软件包对数据进行统计学分析。结果:59/167(35.3%)例病例中检测到RASSF1A基因启动子区甲基化。101/167(60.5%)例病例中RASSF1A蛋白呈低或不表达,66/167(39.5)病例中RASSF1A蛋白呈高表达。存在RASSF1A基因甲基化组,RASSF1A蛋白表达显著低于不存在甲基化组(P=0.012)。去甲基化药物decitabine处理ACC细胞系后,RASSF1A mRNA及蛋白水平表达均升高。结论:唾液腺ACC中,启动子区甲基化是RASSF1A基因失活的主要原因,可作为该肿瘤的潜在治疗靶点。PURPOSE： To investigate the relationship between RASSF1 A expression and promoter methylation in salivary adenoid cystic carcinoma（ACC）. METHODS： One hundred and sixty-seven tumor tissues of ACC and 50 gland tissues were analyzed for promoter methylation status of RASSF1 A by bisulfite sequencing PCR（BSP） and/or methylation specific PCR（MSP）, and for protein expression by immunohistochemistry. The promoter methylation status and expression of RASSF1 A were detected before and after treated with decitabine in SACC-83. The data was analyzed with SPSS 18.0 software package.RESULTS： Promoter methylation of RASSF1 A was found in 35%（59/167） of ACC tissues. Sixty-six ACC cases showed higher protein expression of RASSF1 A and 101 cases had no/lower expression. Lower/no expression of RASSF1 A was correlated with promoter methylation of RASSF1A（P=0.012）. After treated with decitabine in SACC-83, mRNA and protein expression of RASSF1 A was promoted. CONCLUSIONS： Promoter methylation of RASSF1 A is the main mechanism for inactivation of RASSF1 A in salivary ACC and may be as a potential therapeutic target of treatment of this tumor.

关 键 词：腺样囊性癌 RASSF1A DNA甲基化 蛋白表达 

分 类 号：R739.87[医药卫生—肿瘤]