环瓜氨酸肽辅助完全弗氏佐剂致关节炎大鼠的模型研究  

The model research of arthritis rat induced by Complete Freund's adjuvant combining with cyclic citrullinated peptide

作　　者：巨少华[1,2] 胡勇[1,2] 任香怡 代渊[2] 陈欢[1,2] 魏江平[1,2] 徐世军[1,2] 

机构地区：[1]四川省中药资源系统研究与开发利用国家重点实验室培育基地,成都611137 [2]成都中医药大学,成都611137

出　　处：《中药药理与临床》2014年第4期135-137,共3页Pharmacology and Clinics of Chinese Materia Medica

基　　金：国家自然科学基金面上项目(81273900);中药药理四川省青年科技创新研究团队(2014TD0007)

摘　　要：目的:研究环瓜氨酸肽(CCP)辅助完全弗氏佐剂(CFA)造模对佐剂性关节炎模型大鼠临床体征、血清因子、病理的影响。方法:SD大鼠随机分为4组,即空白对照组、完全弗氏佐剂雄性组(雄CFA组)、完全弗氏佐剂雌性组(雌CFA组)、完全弗氏佐剂+环瓜氨酸肽组(CCP+CFA组)。除空白对照组注射0.1ml生理盐水外,雄CFA组和雌CFA组注射0.1ml CFA,CCP+CFA组注射含有100μg环瓜氨酸肽的0.1ml CFA,造模后每隔3d观察大鼠足肿胀度,第28d处死取血清ELISA法检测IL-6、TNF-a、RF、anti-CCP,致炎侧踝关节进行病理检测。结果:与空白对照组比较,雄CFA组、雌CFA组和CCP+CFA组大鼠关节肿胀度在各个时间段均显著增大,但各模型组之间无显著的统计学意义;与空白对照组比较,各模型组IL-6、TNF-a、RF、anti-CCP均明显升高,具有极显著的统计学意义,与雄CFA组比较,CCP+CFA组IL-6、TNF-a、anti-CCP含量均升高,具有显著的统计学意义,但雌、雄CFA组之间无显著差异;与空白组比较,各模型组炎性细胞浸润、滑膜组织增生、纤维组织增生、巨噬细胞增生、骨质侵蚀水平均明显升高,具有显著的统计学意义,但各模型组之间无统计学意义。结论:环瓜氨酸肽作为一种类风湿关节炎造模的辅助剂,其血清IL-6、TNF-a、RF、anti-CCP均较普通模型显著,可以应用到类风湿关节炎的研究中。To study the clinical signs, serum factors and pathology of adjuvant arthritis rats, which were induced by complete freand- adjuvant （CFA） with cyclic cindlinated peptide （CCP）. Method： Sprague-Dawley rats were randomly divided into four groups： control, male complete freund＇s adjuvant（maleCFA）, female complete freund＇s adjuvant（ femaleCFA）, complete freund＇s adjuvant with cyclic cirullinated peptide（ CCP ＋ CFA）. maleCFA and femaleCFA were induced by 0. lml CFA. CCP ＋ CFA were induced by 0.1 ml CFA with 100μg CCP. Control animals were similarly injected with saline. During the experimental period, paw swelling was regularly measured every 3 days. All animals were sacrificed on the 28th day after injection, the serum IL-6, TNF-a, RF and anti-CCP levels were measured by ELISA kits, and hind preinflammatory ankle for pathological examination. Result： Compared with the control group, all model groups ＇paw swelling obviously increased on every periods（ P 〈0.001 ）, but the models were no significant among them（ P 〉 0. 05）. The serum IL--6, TNF-a, RF and anti- CCP levels of all model groups increased contrasting with the control group（ P 〈0. 001 ）. The IL-6, TNF-a, RF and anti-CCP of CCP ＋ CFA group notably increased comparing with maleCFA group（ P 〈0.05）, but the maleCFA and femaleCFA were not significant between them（ P 〉 O. 05）. Inflammatory cell infiltration, synovial hyperplasia, fibrosis, macrophage proliferation and bone erosion levels of all model groups obviously increased contrasting with the control group（P 〈 0.01 ）, but the models have no significance among them（ P 〉 0.05）. Conclu- sion： CCP is an adjuvant for rheumatoid arthritis, mixing with CFA which makes significant contributions to the serum IL-6, TNF-a, RF and anti-CCP levels, and can be used for the experimental study of rheumatoid arthritis.

关 键 词：环瓜氨酸肽 佐剂性关节炎模型 

分 类 号：R-332[医药卫生] R593.22