骨诱导膜活化灭活骨修复骨缺损的实验研究  

Inactivated bone graft activated by bone induced membrane repairs bone defect in rabbits

作　　者：汪小华[1] 魏富达 喻胜鹏 李伟[1] 黄科[1] 董世武[2] 谢肇[1] 

机构地区：[1]第三军医大学西南医院骨科,国家地方联合工程实验室,全军矫形外科中心,重庆400038 [2]第三军医大学生物工程学院生物医学材料学教研室,重庆400038

出　　处：《第三军医大学学报》2014年第20期2061-2064,共4页Journal of Third Military Medical University

基　　金：国家自然科学基金面上项目(30973065);创伤;烧伤与复合伤国家重点实验室开放基金(SKLKF201313)~~

摘　　要：目的构建骨缺损诱导膜模型,探讨骨诱导膜对灭活移植骨成骨诱导活性的作用。方法 32只新西兰大白兔一期在桡骨中段制造骨缺损模型,植入聚甲基丙烯酸甲酯骨水泥诱导生物膜形成。第6、8周分别处死4只取出诱导膜检测骨形态发生蛋白(bone morphogenetic protein-2,BMP-2)和血管内皮生长因子(vascular endothelial growth factor,VEGF)含量并切片作HE染色,取同只兔皮下组织作为对照。其余24只兔在一期植入骨水泥后6周取出骨水泥进行二期手术,按随机数字表法分为实验组、模型组和对照组,每组8只。实验组保留诱导膜并在膜内植入灭活骨,模型组保留诱导膜不植骨,对照组去除诱导膜植入灭活骨。二期处理后第8、12周通过放射学和组织学评估新生骨形成情况。结果ELISA检测表明在第6、8周诱导膜中BMP-2含量[(437.40±20.75)、(346.67±30.05)pg/mL]、VEGF含量[(362.33±38.85)、(233.84±18.20)pg/mL]均高于皮下组织(P<0.05),同时在诱导膜切片HE染色中观察到软骨内骨化;放射学检测可见二期处理后第8、12周实验组成骨优于模型组和对照组(P<0.01),模型组有少许骨痂形成,对照组无成骨;第8、12周组织学观察实验组可见骨细胞和软骨细胞形成,成骨优于模型组和对照组(P<0.01),对照组仅有纤维连接,未见骨细胞和软骨细胞。结论骨诱导膜含有间充质干细胞和高浓度的细胞因子,从而促进灭活移植骨重建骨缺损。Objective To construct a bone defect-induced membrane model and to discuss the osteogenic activity of the bone induced membrane on inactivated bone graft.Methods A radial defect model was built with 32 New Zealand rabbits.Polymethyl methacrylate（ PMMA） bone cement spacer was inserted into the defect to induce membrane.After 6 and 8 weeks,8 rabbits（ 4 for each time） were sacrificed and bone induced membranes were taken out to test bone morphogenetic protein-2（ BMP-2） and vascular endothelial growth factor（ VEGF） levels and to perform HE staining of sections,with rabbit subcutaneous tissue as control.Bone cement was taken out from the other 24 rabbits in 6 weeks after implantation for secondary operation,including 3 groups,an experimental group [keeping the induced membrane in situ and implanting autologous iliac inactivated with high temperature water（ 100 ℃ 30 min） ],a model group（ keeping the induced membrane in situ）,and a control group（ removing the induced membrane and implanting autologous iliac inactivated with high temperature water）.Radiological and histological evaluation of the new bone formation was done at 8 and 12 weeks.Results（ 1） BMP-2 and VEGF levels in the induced membrane were 437.40 ±20.75 pg /mL and 362.33 ± 38.85 pg /mL,respectively,at 6 weeks and 346.67 ± 30.05 pg /mL and233.84 ± 18.20 pg /mL,respectively,at 8 weeks,higher than those of the subcutaneous tissue（ P〈0.05）,while endochondral ossification was observed in induced membrane slice.（ 2） Radiological detection at 8 and 12 weeks after the secondary operation indicated that bone formation was more in the experimental group than in the model group and the control group（ P〈0.01）.There was a little callus formation in the model group,while the control group had no bone formation.（ 3） Histological detection at 8 and 12 weeks after the secondary operation indicated that bone formation was more in the experimental group than in the model group and the control group（ P〈0.01）.Only f

关 键 词：诱导膜 骨重建 灭活骨 间充质干细胞 

分 类 号：R-332[医药卫生] R364.3