麻疹-流行性乙型脑炎联合减毒活疫苗猴体神经毒力研究  

Neurovirulence of Measles and Japanese Encephalitis Combined,Attenuated,Live Vaccine in Rhesus Macaques (Macaca mulatta)

作　　者：李萍萍[1] 李伟[2] 刘胜[1] 屈哲[2] 齐卫红[2] 贾丽丽[3] 易玲[1] 王月[1] 

机构地区：[1]武汉生物制品研究所有限责任公司,国家联合疫苗工程技术研究中心,湖北武汉430207 [2]中国食品药品检定研究院/国家药物安全评价监测中心,北京100176 [3]中国食品药品检定研究院,北京100050

出　　处：《中国疫苗和免疫》2014年第5期398-402,共5页Chinese Journal of Vaccines and Immunization

基　　金：国家高技术研究发展计划(863计划)重大项目生物和医药技术领域疫苗与抗体工程项目(编号2006AA02A233);生物技术领域疫苗研发关键技术及产品研发项目(编号2012AA02A401)

摘　　要：目的观察恒河猴对麻疹-流行性乙型脑炎(乙脑)联合减毒活疫苗(Measles and Japanese Encephalitis Combined Attenuated Live Vaccine,M-JEV)的神经毒力产生及其严重程度,对M-JEV进行安全性评价。方法猴两侧丘脑和腰髓内注射M-JEV,同时设阴性对照及麻疹减毒活疫苗和乙脑减毒活疫苗对照。观察临床症状,并检查注射部位、体温、血液学、血液生物化学、摄食量、眼科学以及血清抗体等变化情况;实验结束进行大体病理学和组织病理学检查。结果各项观察指标及检查结果均与对照疫苗或阴性对照相似,相关数据与对照疫苗或阴性对照组比较差异均无统计学意义。结论 M-JEV表现出与单价疫苗相似的安全性。Objective Observe characteristics and severity of neuronal lesions in the Rhesus Macaque neurovirulence test of measles and Japanese encephalitis combined attenuated live vaccine（ M-JEV） to evaluate safety of M-JEV.Methods Rhesus Macaques were administered M-JEV by thalamus and lumbar cord intra-spinal injections,and were compared with non-vaccinated（ negative control） and measles-vaccinated and Japanese encephalitis-vaccinated Rheses Macaques.We observed clinical symptoms and examined the injection sites,body temperature,hematology indicators,blood biochemistry indicators,food intake,ophthalmological indicators,and serum antibody levels.We conducted macropathological and histopathological examinations after the neurovirulence test.Results All test results from the M-JEVvaccinated Rhesus Macaques were similar to those from negative controls,measles-vaccinated and Japanese encephalitis-vaccinated Rhesus Macaques,with no statistical differences found.Conclusion M-JEV performed similarly to monovalent vaccines with respect to vaccine safety.

关 键 词：麻疹-流行性乙型脑炎联合减毒活疫苗 安全性评价 猴 神经毒力 

分 类 号：R186[医药卫生—流行病学] R511.1[医药卫生—公共卫生与预防医学]