Global Insight into N-Glycome and N-Glycoproteome of Three Most Abundant Snake Venoms in Asia  

作　　者：CAO Weiqian HUANG Jiangming CAO Jing YANG Pengyuan 

机构地区：[1]Institutes of Biomedical Sciences and Department of Chemistry, Fudan University, Shanghai 200433, P. R. China

出　　处：《Chemical Research in Chinese Universities》2014年第5期726-730,共5页高等学校化学研究（英文版）

基　　金：Supported by the National Basic Research Program of China（No.2013CB911201）, the National High Technology Research and Development Program of China（No.2012AA020200）, the National Natural Science Foundation of China（No.31100590） and the Shanghai Municipal Natural Science Foundation, China（No. 11ZR1403000）.

摘　　要：Snake venom is a complex cocktail including a variety of biological active proteins and proteinaceous components, which have considerable medical and pharmacological importance. N-Glycosylation is widely impli- cated as a common modification in numerous venom proteins and impacts the in vivo venomic functions. However, systematic survey of N-glycome and N-glycoproteome on snake venoms has not been undertaken. In this study, em- ploying combination of N-glycomics and N-glycoproteomics strategies, we explored the N-glycosylation including both N-glycoproteins and N-glyco-chains in three venoms from Agkistrodon blomhoffii, Naja naja atra Cantor and Vipera russelii siamensis Smith, respectively, which are amongst the most abundant venomous snakes in Asia. As a result, numbers of N-glycoproteins and N-glycans were identified. However, the overlaps of N-glycoproteins and N-glycans of the three venoms were small. Thus, the exploration results of N-glycome and N-glycoproteome indicate that N-glycosylation increases the complexity and variety of the three venoms. Our research provided some new horizons for the comprehensive understanding of venoms variation, which is helpful for the basic venom re- search as well as the management of snake envenomation.Snake venom is a complex cocktail including a variety of biological active proteins and proteinaceous components, which have considerable medical and pharmacological importance. N-Glycosylation is widely impli- cated as a common modification in numerous venom proteins and impacts the in vivo venomic functions. However, systematic survey of N-glycome and N-glycoproteome on snake venoms has not been undertaken. In this study, em- ploying combination of N-glycomics and N-glycoproteomics strategies, we explored the N-glycosylation including both N-glycoproteins and N-glyco-chains in three venoms from Agkistrodon blomhoffii, Naja naja atra Cantor and Vipera russelii siamensis Smith, respectively, which are amongst the most abundant venomous snakes in Asia. As a result, numbers of N-glycoproteins and N-glycans were identified. However, the overlaps of N-glycoproteins and N-glycans of the three venoms were small. Thus, the exploration results of N-glycome and N-glycoproteome indicate that N-glycosylation increases the complexity and variety of the three venoms. Our research provided some new horizons for the comprehensive understanding of venoms variation, which is helpful for the basic venom re- search as well as the management of snake envenomation.

关 键 词：GLYCOSYLATION N-Glycomics N-Glycoproteomics Snake venom 

分 类 号：Q53[生物学—生物化学] Q959.620.6