三七总皂苷对局灶性脑梗死大鼠海马CA1区NogoA/NgR_1/RhoA信号通路蛋白表达的影响  

Effect of Totalsaponins of Panax Notognseng Injection on the Expression of Nogo-A/Ngr/Rhoa Signaling Regulation Pathway in Hippocampus CA1 in Rats with Middle Cerebral Artery Occlusion

作　　者：刘炜[1] 刘丽星[2] 史晓伟[1] 张晓萌[1] 施怡[1] 朱陵群[1] 

机构地区：[1]北京中医药大学东直门医院中医内科学教育部重点实验室和北京市重点实验室,北京100700 [2]首都医科大学附属北京地坛医院顺义院区中医科,北京100015

出　　处：《中西医结合心脑血管病杂志》2014年第11期1370-1373,共4页Chinese Journal of Integrative Medicine on Cardio-Cerebrovascular Disease

基　　金：国家自然科学基金资助项目(No.30772803;81173235)

摘　　要：目的探讨三七总皂苷(total saponins of panax noto ginseseng,PNS)对局灶性脑梗死大鼠海马CA1区神经元保护及促进神经重塑的机制。方法将动物随机分为Y27632组、PNS加Y27632组、假手术组和手术组,对除了假手术组外的所有动物,建立大鼠大脑中动脉阻塞(MCAO)模型,造模成功且未分组的动物根据Longa评分和体重进行随机分组,分别为模型组、PNS组、尼莫地平组。观察造模7d后大鼠一般活动状态、体重指数和海马CA1区神经元形态学及Nogo-A、NgR1和RhoA蛋白表达变化。结果与假手术组比较,模型组Nogo-A、NgR1和RhoA蛋白表达增多(P<0.01);与模型组比较,PNS组大鼠Nogo-A、NgR1和RhoA蛋白表达均下降(P<0.05),PNS加Y27632组NgR1和RhoA蛋白表达降低(P<0.05),Y27632组RhoA蛋白表达下降(P<0.01)。结论 PNS具有与Y27632相似的作用,对脑梗死后Nogo-A/NgR1/Rho通路的上下游均有调节作用,可促进脑梗死损伤后海马神经功能重塑。O bjective To study the effect o f to ta lsaponins o f panax no tognseng(PNS) injection on the rem o ldability and pro tec-tive m echanism in hippocam pus CA1 in ra ts w ith m iddle cerebra l artery occlusion (MCAO). M e thods The Y27632 g roup,PNS and Y27632 g roup and the contro lg roup w ere taken out random ly from a l SPF S prague D aw ley ra ts. A l o f the ra ts w ere used to estab-lish MCAO m ode l by im proving Longa M e thod except to the contro l g roup. Acco rding to Longa sco res and w e ights the successful m ode led and un g roup ra ts w ere random ly divided into m ode lg roup,PNS g roup and nim odipine(NP) g roup.A l o f ra ts w ere execu-ted 7 days after opera tion. The genera l sta te, w e ight and neuro log ica l function sco re o f the ra ts w ere assessed da ily.The pa tho log i-ca l and m o rpho log ica l and the expression o fNogo A, NgR1 and RhoA in hippocam pus CA1 w ere de tected.Results The expres-sion o fNogo A, NgR1 and RhoA in m ode l g roup w ere m o re than the contro l g roup P < 0.01). The expression o f Nogo A, NgR1 and RhoA in PNS g roup and the expression o fNgR1 and RhoA in PNS and Y27632 gourp w ere less than the modelgroup( P < 0.05 or( P< 0.01). The expression o fRhoA in Y27632 g roup w ere low er than the m ode lg roup( P < 0.01). Conclusion The PNS injection has the sam e effects as Y27632 and regula tes bo th the upstream and dow nstream o fNogo A /NgR1/RhoA signa ling pa thw ay, so it can enhance the recovery o f hippocam pus CA1 neuro log ica l function o f the MCAO ra ts.

关 键 词：局灶性脑梗死 三七总皂苷 海马 神经重塑 NogoA 

分 类 号：R743.3[医药卫生—神经病学与精神病学]