Inhibin、CD99、Calretinin、WT1和Melan-A在卵巢粒层细胞瘤中的免疫组织化学表达  

Immunohistochemical expression of Inhibin, CD99, Calretinin, WT1 and Melan-A in granulosa cell tumor

作　　者：叶薇[1] 薛德彬[1] 陈智伟[1] 肖芳[1] 杨丽英[1] 陈玉琳[1] 陈东[2] 

机构地区：[1]福建省莆田学院附属医院病理科,福建莆田351100 [2]浙江省宁波市妇女儿童医院病理科,浙江宁波315000

出　　处：《中国处方药》2014年第11期1-3,共3页Journal of China Prescription Drug

摘　　要：目的检测多种免疫组织化学标记物在卵巢粒层细胞瘤(GCT)的表达情况,结合临床资料及组织形态学特征讨论此瘤与卵巢支持-间质细胞瘤(SLCT)的鉴别诊断。方法收集22例GCT(成年型18例,幼年型4例)与13例SLCT(高分化6例,中分化7例),检测5种常用的卵巢性索间质肿瘤免疫组织化学标记物(包括Inhibin、CD99、calretinin、WT1和melan-A)以及其他多种非特异性标记物(包括CK5/6、CK18、EMA、Vimentin、SMA、S-100、CD10、ER、PR、AR、p53、Ki67)在这两种肿瘤中的表达特征。结果在GCT的粒层细胞,Inhibin的表达率最高(94%),而WT1的表达率最低(78%);而在GCT的间质细胞,WT1的表达率最高(100%),CD99的表达率最低(阴性)。其它非特异性标记物在GCT和SLCT中表达的敏感性和特异性均较低。结论结合临床特征与组织学特点,用Inhibin、CD99、calretinin、WT1和melan-A作为一组抗体,有助于GCT与SLCT的鉴别诊断。Objective Immunohistochemical markers were determined in GCT, and the distinction of GCT and SLCT was discussed together with clinical data and histomorphology. Methods Twenty-two cases of GCTs and 13 cases of SLCTs were selected. Five common markers of the ovarian sex cord stromal tumors(including Inhibin, CD99, calretinin, WT1 and melan-A) and many other non-specific markers(including CK5/6, CK18, EMA, Vimentin, SMA,S-100, CD10, ER, PR, AR, p53, Ki67) were determined immunohistochemically. Results Inhibin and WT1 represtented the highest and lowest expression rate(94% and 78%, respectively) in the granulosa cells of GCT, meanwhile, WT-1 and CD99 showed the highest and lowest expression rate(100% and negative, respectively) in the stromal cells of GCT. Conclusion A panel of immunohistochemical markers including Inhibin, CD99, calretinin, WT1 and melan-A together with clinical data and histomorphology was very helpful for the differential diagnosis of GCT and SLCT.

关 键 词：卵巢 粒层细胞瘤 支持一间质细胞瘤 免疫组织化学 鉴别诊断 

分 类 号：R737.31[医药卫生—肿瘤]