人乳头瘤病毒与宫颈癌患者循环血中微小RNA218相关性研究  

The correlation between human papillomavirus and microRNA218 in circulating blood of patients with cervical carcinoma

作　　者：张玢玢[1] 闫翠云[1] 葛虹 魏涛[1] 梁国伟[1] 薛改莲 郭亮[3] 程丽[3] 孙立新[4] 

机构地区：[1]太原钢铁(集团)有限公司总医院妇产科,030003 [2]山西医药杂志社 [3]太原钢铁(集团)有限公司总医院中心实验室,030003 [4]山西省肿瘤医院妇科

出　　处：《中国药物与临床》2014年第10期1326-1328,共3页Chinese Remedies & Clinics

基　　金：太原市科学技术发展计划(12016913)

摘　　要：目的探讨人乳头瘤病毒(HPV)与宫颈癌患者循环血中微小RNA(miRNA)218的关系。方法收集宫颈癌患者作为病例组,选取年龄与病例组匹配并符合对照组标准的子宫肌瘤作为对照组。采血并测定对照组和病例组循环血中的miRNA218水平,分析病例组中不同HPV亚型感染患者的血miRNA218表达水平;以及不同病理分期宫颈癌患者的血miRNA218表达水平。结果病例组患者中HPV16亚型和HPV18亚型感染的患者血中miRNA218表达水平明显低于对照组和病例组HPV其他亚型感染者(P<0.01);宫颈癌Ⅰ期患者血中miRNA218表达水平明显低于对照组(P<0.01);并且宫颈癌Ⅱ期患者血中miRNA218表达水平明显低于宫颈癌Ⅰ期(P<0.05)和对照组(P<0.01)。结论循环血中miRNA218水平和宫颈癌侵袭程度密切相关;并且高危HPV亚型HPV16和HPV18感染患者与循环血中miRNA218水平密切相关。Objective To investigate the correlation between human papillomavirus （HPV） and microRNA （miRNA） 218 in circulating blood of patients with cervical carcinoma. Methods Cervical carcinoma patients were re-cruited as the case group, and female patients with hysteromyoma matching for the age of case group were recruited as control group. MiRNA218 levels in the circulating blood were determined, and the miRNA218 expression in circulat-ing blood of patients with cervical carcinoma patients infected with different types of HPV were analyzed. The miR-NA218 levels in cervical carcinoma patients with different pathologic stages were also analyzed. Results The expres-sion of miRNA218 in circulating blood of patients with cervical carcinoma infected with HPV subtype-16 and subtype-18 were significantly attenuated compared with those in control group and cervical carcinoma patients infected with other subtypes of HPV （both P〈0.01）. Moreover, the miRNA218 expression level in circulating blood of patients with stage Ⅰ cervical carcinoma was significantly lower than that of the normal control group （P〈0.01）. Additionally, the miRNA218 expression level in circulating blood of patients with stage Ⅱ cervical carcinoma was significantly attenu-ated than that of case group （P〈0.05） and control group （P〈0.01）. Conclusion The miRNA218 levels in circulating blood are closely correlated with tumor invasion in cervical carcinoma patients. High-risk HPV, namely the subtype-16 and subtype-18, are closely correlated with the miRNA218 levels in circulating blood of patients with cervical carcino-ma.

关 键 词：人乳头瘤病毒 16 人乳头瘤病毒 18 宫颈肿瘤 微小 RNA218 

分 类 号：R737.33[医药卫生—肿瘤]