Octamer 4基因在非小细胞肺癌中的表达及临床意义  

Expression and clinical significance of Octamer 4 in non-small cell lung cancer

作　　者：杨玉清[1] 姚宏[2] 白家璐 魏俊燕 郭亮[3] 王菲 李媛 李建民[4] 

机构地区：[1]太原钢铁(集团)有限公司总医院病理科,030003 [2]太原市人民医院病理科 [3]太原钢铁(集团)有限公司总医院中心实验室,030003 [4]山西省肿瘤医院病理科

出　　处：《中国药物与临床》2014年第10期1332-1335,共4页Chinese Remedies & Clinics

基　　金：太原钢铁(集团)有限公司总医院科研课题(2013-8)

摘　　要：目的检测Octamer 4(Oct-4)基因在非小细胞肺癌(NSCLC)中的表达,探讨Oct-4在NSCLC发生、发展中的作用。方法采用流式细胞仪分选出肺癌细胞系A549中Oct-4+及Oct-4-的细胞群,用免疫荧光法检测分选后的细胞亚群中CD133的表达情况;采用体外侵袭实验检测Oct-4+细胞群和Oct-4-细胞群的侵袭能力。结果 1以标记Oct-4通过流式细胞仪分离后,成功获得肺癌细胞株A549中Oct-4+细胞群和Oct-4-细胞群。且Oct-4+细胞群中CD133的表达明显高于Oct-4-细胞群,差异有统计学意义(P<0.05)。2Oct-4+细胞群的侵袭细胞数显著高于Oct-4-细胞群,差异有统计学意义(P<0.05)。3Oct-4在NSCLC中的表达率为83%(50/60),且Oct-4+细胞群中CD133的表达明显高于Oct-4-细胞群,两者比较差异有统计学意义(P<0.05);且Oct-4的表达与NSCLC组织的分化程度、肿瘤的临床分期及是否已经发生淋巴结转移有关,差异有统计学意义(P<0.05)。结论干细胞标志物Oct-4在NSCLC干细胞亚群中呈高表达,可能与肺癌的发生、发展及转移密切相关,为肺癌治疗提供新的靶点。Objective To detect the expression of Octamer 4 （Oct-4） in non-small cell lung cancer （NSCLC）, and to explore its role in the pathogensis and development of NSCLC. Methods Oct-4＋ and Oct-4- cells in lung can-cer cell line A549 were selected by using flow cytometry, and the expression of sorted cells CD133 were detected by immumofluorescence assay. Invasion assay in vitro was adopted to detect the invasive capacity of Oct-4＋ cell subsets and Oct-4- cell subsets. Results After separation of Oct-4 labeled with flow cytometry, Oct-4＋ cell subsets and Oct-4-cell subsets in lung cancer cell line A549 were obtained. The expression of CD133 in Oct-4＋ cell subsets was signifi-cantly higher than that of Oct-4- cell subsets （P〈0.05）. The number of invasion cells of Oct-4＋ cell subsets was signifi-cantly higher than that of Oct-4- cell subsets （P〈0.05）. The expression rate of Oct-4 in NSCLC was 83% （50/60）. The expression of CD133 in Oct-4＋ cell subsets was significantly higher than that in Oct-4- cell subsets （P〈0.05）. The ex-pression of Oct-4 was related with the differentiation of NSCLC tissue, clinical staging of tumor and the occurrence of lymph node metastasis （P〈0.05）. Conclusion The stem cell markers Oct-4 showed high expression in NSCLC stem cell subpopulations, which may be closely associated with the pathogenesis, development and metastasis of lung can-cer, thus offering a novel target for the treatment of lung cancer.

关 键 词：癌 非小细胞肺 干细胞 OCT-4 

分 类 号：R734.2[医药卫生—肿瘤]