莱菔硫烷抑制人胃癌SGC7901细胞增殖及其诱导凋亡分子流行病学研究  

Effect of sulforaphane on anti-proliferation and apoptotic induction of human gastric cancer cell line SGC7901

作　　者：贾战平[1] 李大鹏[1] 王晓磊[2] 鲍春丹[1] 王滨有[1] 

机构地区：[1]哈尔滨医科大学公共卫生学院流行病教研室,黑龙江哈尔滨150081 [2]哈尔滨学院实验教学中心,黑龙江哈尔滨150086

出　　处：《中华疾病控制杂志》2014年第10期931-934,共4页Chinese Journal of Disease Control & Prevention

摘　　要：目的研究莱菔硫烷（sulforaphane,SFN）在体外对人胃癌细胞SGC7901的增殖抑制作用,探讨SFN诱导细胞凋亡的分子机理。方法以SFN和人胃癌细胞SGC7901为研究对象。采用四甲基偶氮唑盐比色法观察不同浓度SFN对细胞增殖的抑制影响,AO/EB双重染色法与透射电镜进行形态学变化观察,琼脂糖凝胶电泳观察细胞凋亡状态下的DNA变化,RT-PCR检测细胞p21基因mRNA表达。结果 50～150μmol/L浓度范围的SFN对SGC7901细胞的增殖有明显的抑制作用。AO/EB染色结果显示SFN诱导SGC7901细胞凋亡发生形态学上改变。DNA琼脂糖凝胶电泳显示细胞经SFN（100μmol/L、150μmol/L）处理24 h后可见＂梯形＂DNA碎片条带。RT-PCR结果显示50μmol/L SFN能够诱导p21基因mRNA的表达增强。透射电镜观察到SFN作用SGC7901细胞48 h后出现核固缩等早期凋亡镜下改变。结论 SFN能抑制SGC7901细胞的增殖并诱导SGC7901细胞凋亡,可在翻译和转录水平上调p21的表达,其分子机理可能与调控p21基因存在关系。Objective To investigate the anti-proliferative effect of sulforaphane on human gastric cancer cell line SGC7901 in vitro and the molecular mechanism of cell apoptosis induced by sulforaphane. Methods Sulforaphane and human gastric cancer cell line SGC7901 were used for the study. MTT assay was used to observe the inhibition of cell proliferation on different concentrations of sulforaphane. AO/EB double staining and transmission electron microscope were applied to observation of morphological changes of apoptotic cells. The DNA fragmentation of apoptosis cells was examined by agarose gel electrophoresis. RT-PCR detected the expression of p21 gene mRNA. Results Sulforaphane in 50-150txmol/L concentration range could significantly suppress the proliferation of SGC7901 cells in a dose-dependent manner by MTY assay. AO/EB double staining showed the typical apoptotic morphological changes induced by sulforaphane. Exposing to sulforaphane （100p, mol/L, 1501~mol/L） for 24h, the formation of DNA Ladder was detectable in agarose gel electrophoresis. RT-PCR showed 50μmol/L sulforaphane could upregulate the p21 mRNA level in SGC790t cells. Typical early apoptosis morphology changes of SGC7901 cells were notable in transmission electron microscope exposing to sulforaphane for 48h. Conclusion Sulforaphane can inhibit the proliferation, induce apoptosis, and up-regulate the transcription and translation expression of p21 gene in SGC7901 cell line. The molecular mechanism is possibly related to the modulation of the level of p21 mRNA expression.

关 键 词：莱菔硫烷 胃肿瘤 分子流行病学 

分 类 号：R735.2[医药卫生—肿瘤]