乳腺癌患者TCR BV、BJ限制性取用及序列分析  

Analyses of the restricted usage of TCR BV, BJ subfamily and their sequences in patients with breast cancer

作　　者：张建波[1] 吕晓东[2] 于庆凯[1] 宋永平[3] 胡骏[4] 

机构地区：[1]郑州大学附属肿瘤医院河南省肿瘤医院病理科,450003 [2]郑州大学附属肿瘤医院河南省肿瘤医院中心实验室,450003 [3]郑州大学附属肿瘤医院河南省肿瘤医院血液科,450003 [4]中山大学中山医学院微生物学教研室

出　　处：《肿瘤研究与临床》2014年第9期605-607,共3页Cancer Research and Clinic

基　　金：河南省基础与前沿技术研究计划（102300410038）

摘　　要：目的 分析乳腺癌TCR BV、BJ亚家族限制性取用及序列分析.方法 选取乳腺癌转移淋巴结,提取RNA后反转录,多重PCR扩增TCR β全长序列,构建重组质粒并测序,利用网上TCR资源分析序列.结果 2例患者共获得5个TCR β链全长序列.BV亚家族分别限制性取用BV 2、BV 14、BV29S1,BJ亚家族分别限制性取用BJ1S1、BJ2S2、BJ2S3、BJ2S5.序列分析增生T细胞克隆CDR3区有不同的氨基酸序列.结论 乳腺癌患者TCR BV、BJ亚家族具有限制性取用,不同T细胞克隆的CDR3序列不同.Objective To analyze the restricted usage of TCR BV,BJ subfamily and their sequences in patients with breast cancer.Methods The total RNA was extracted from tissues of lymph node metastasis,then reverse transcripted.The complete DNA sequence of TCR β-chain was amplified by multi-PCR.The recombinant plasmids were sequenced by using online TCR resources.Results 5 TCR β-chain CDS were obtained in two patients.TCR β-chain presented specific repertoire skewing in metastatic lymph node.There were selected usage of BV 2,BV 14,BV 29S1 of BV subfamily and BJ1S1,BJ2S2,BJ2S3,BJ2S5 of BJ subfamily.The clonal proliferation T cells had different CDR3 amino acid sequences.Conclusions There are selected usages of TCR BV,BJ subfamily in patients with breast cancer.The sequences of CDR3 are mostly different in TCR clone proliferation.

关 键 词：乳腺肿瘤 多重聚合酶链反应 互补决定区 

分 类 号：R737.9[医药卫生—肿瘤]