非小细胞肺癌中DLC-1、ROCKI的表达及其临床意义  

Expressions and clinical significances of DLC-1 and ROCK I in non-small cell lung cancer

作　　者：翟玉洁[1] 范庆帅 宁方玲[1] 刘长民[1] 赵大华[1] 陈绍水[1] 

机构地区：[1]滨州医学院附属医院肿瘤科,256600

出　　处：《国际肿瘤学杂志》2014年第9期688-692,共5页Journal of International Oncology

摘　　要：目的探讨肝癌缺失基因-1（DLC．1）和Rho相关卷曲螺旋形成蛋白激酶I（ROCKI）在非小细胞肺癌（NSCLC）中的表达及其临床意义。方法选取48例手术切除并经病理检查证实的NSCLC组织及癌旁组织，采用免疫组织化学EnVision法检测DLC-1、ROCKI蛋白的表达，并分析二者之间的相关性及其与临床病理特征的关系，探讨DLC-1基因对NSCLC患者的预后价值。结果DLC-1蛋白在NSCLC组织中呈低表达或表达缺失，阳性表达率为33．3％（16／48），明显低于癌旁组织的70．8％（34／48），差异有统计学意义（X2=13．523，P〈0．01）；ROCKI蛋白在NSCLC中的阳性表达率为58．3％（28／48），高于癌旁组织的0（0／48），差异有统计学意义（x2=39．529，P〈0．01）；NSCLC中DLC-1的表达与性别、吸烟史、组织类型等无关，与肿瘤分化程度、淋巴结转移、TNM分期有关，且差异有统计学意义；DLC．1阳性表达组中ROCKI的阳性表达率为37．5％（6／16），而ROCKI的阴性表达率为68．8％（22／32），相关分析提示DLC-1与ROCKI在NSCLC中表达呈负相关（r=-2．214，P=0．039）；DLC-1蛋白高表达组3年生存率高于低表达组，差异有统计学意义（P=0．043）。结论DLC-1蛋白的低表达或表达缺失、ROCKI蛋白的表达在NSCLC的发生、发展中可能扮演重要角色，检测DLC-1及ROCKI蛋白的表达，有助于判断NSCLC的生物学行为及预后。Objective To explore the expressions and clinical significances of deleted in liver cancer-1 （DLC-1） and Rho associated coiledcoil forming protein kinase （ROCK） I in non-small lung cancer （NSCLC）. Methods The expressions of DLC-1 and ROCK I in NSCLC and adjacent tissue of 48 patients with pathologically confirmed as NSCLC and undergone surgical resection were detected by immunohistochemistry EnVision method. The correlations among DLC-1 protein, ROCK I protein and the clinical pathological characteristics were analyzed. The prognostic value of DLC-1 in patients with NSCLC was studied. Results The expression of DLC-1 protein in NSCLC tissue was low or missing, and the positive rate was 33. 3% （16/48）, significantly lower than that in the tissue adjacent to carcinoma 70.8% （34/48）, with statistical significance （X2 = 13. 523, P 〈0.01 ）. The positive expression rate of ROCK I protein in NSCLC was 58.3% （ 28/48 ）, higher than that of tissue adjacent to carcinoma 0 （0/48）, with statistical significance （X2 = 39. 529, P 〈 0.01 ）. The expression of DLC-1 protein was correlated with tumor differentiation, lymph node metastasis and clinical stage, rather than with sex, smoking history and organization type. Through the correlation analy-sis, the expression of ROCK I in DLC-1 positive group was 37.5% （6/16）, and the expression rate of ROCK I in DLC-1 negative group was 68.8% （22/32）. There was negative correlation between DLC-1 and ROCK I in NSCLC tissues （r = -2.214, P = 0.039）. The 3 year survival rate. in DLC-1 protein high expression group was obviously higher than that in low expression group, with statistical significance （P = 0. 043 ）. Conclusion Low or missing expression of DLC-1 and high expression of ROCK I protein may play an important role in the occurrence and development of NSCLC. Detecting the expression of DLC-1 and ROCK I protein may be useful for evaluating the biological behavior and prognosis of NSCLC.

关 键 词：癌 非小细胞肺 预后 肝癌缺失基因-1 Pdao相关卷曲螺旋形成蛋白酶I 

分 类 号：R734.2[医药卫生—肿瘤]