二烯丙基二硫化物对膀胱癌细胞p-ERK表达的影响研究  

Study of the influence of p-ERK on DADS to human bladder cancer cells

作　　者：凌峰[1] 王湘莲[2] 欧陵斌[3] 陈敏丰[4] 

机构地区：[1]永州职业技术学院附属医院泌尿微创外科,湖南永州425006 [2]南华大学永州临床学院泌尿外科,湖南永州425006 [3]永州职业技术学院医学检验系,湖南永州425006 [4]中南大学湘雅医院泌尿外科,湖南长沙410008

出　　处：《中国现代医学杂志》2014年第26期32-35,共4页China Journal of Modern Medicine

摘　　要：目的观察大蒜提取物二烯丙基二硫化物对人膀胱癌T24细胞株p-ERK表达的影响,以探讨二烯丙基二硫化物抗膀胱癌的作用,为应用于临床肿瘤的治疗提供部分理论基础。方法用培养的人膀胱癌T24细胞株与二烯丙基二硫化物共孵育培养48 h后,采用逆转录聚合酶链反应(RT-PCR)检测二烯丙基二硫化物对人膀胱癌T24细胞株p-ERK基因转录的影响;并裂解人膀胱癌T24细胞株提取细胞总蛋白,蛋白免疫印迹检测p-ERK蛋白的表达情况。结果人膀胱癌T24细胞株与二烯丙基二硫化物共孵育后,p-ERK基因表达mRNA的水平明显降低(P<0.05),而对照组表达水平无明显变化(P>0.05);人膀胱癌T24细胞株与二烯丙基二硫化物共孵育后即可检测出p-ERK蛋白的表达减少(P<0.05),而对照组表达水平无明显变化(P>0.05)。结论二烯丙基二硫化物可下调人膀胱癌T24细胞株p-ERK基因的转录与p-ERK蛋白的表达,这可能是二烯丙基二硫化物抗癌的机制之一。[ Objectives] To observe the expression of p-ERK in human bladder cancer after handled by traditional Chinese medicine Sophora extract DADS, and to study the whether the p-ERK gene transcription in cultured human bladder cells is affected by DADS. Then provide some theoretical basis and used terms to investigate the anti-tumor mechanism and clinical tumor therapy of DADS. [Methods] The cultured human bladder ceils were incubated with DADS for 48 h, p-ERK gene transcription on Bladder cells was detected by RT- PCR after incubated with DADS. Western blot was used to detect expression of p-ERK, after bladder cell was cracked and total protein was extracted; p-ERK protein expression in bladder cells were incubated with DADS. [Results] Bladder cells were incubated with DADS, RT-PCR shows the mRNA level of p-ERK decreased when bladder cells was incubated with DADS for 48 h, but there was no marked change in control group; when bladder cells had been infected for 48 h, the expression of p-ERK was suppressed. [Conclu-sion] DADS can reduce p-ERK gene expression, this may be one of the DADS anticancer mechanism.

关 键 词：二烯丙基二硫化物 人膀胱癌T24细胞株 P-ERK 

分 类 号：R737.14[医药卫生—肿瘤]