UPLC-MS/MS法测定液态乳中舒巴坦残留  

Determination of sulbactam residues in milk by UPLC-MS/MS method

作　　者：崔向云[1] 常建军[1] 张雪峰[1] 李照[1] 袁凤琴[1] 徐向峰[1] 宋晓东[1] 

机构地区：[1]内蒙古蒙牛乳业(集团)股份有限公司中心实验室,呼和浩特011500

出　　处：《中国乳品工业》2014年第10期42-43,45,共3页China Dairy Industry

摘　　要：建立了牛乳中舒巴坦的超高效液相色谱-串联质谱（UPLC-MS/MS）检测法。牛乳中的目标物经体积分数为0.2%乙酸-水溶液提取后,经HLB固相萃取柱净化、浓缩,再用超高效液相色谱-串联质谱仪进行测定,外标法定量。方法的回收率为83.3%～116.3%,且相对标准偏差为1.9%～8.6%。该方法快速、准确,回收率高,可用于牛乳中舒巴坦的快速检测。The paper establishes a method to determine sulbactam content in milk using UPLC-MS/MS. Acetic acid solution was added to milk samples to extract target ingredient, and then concentrated by HLB SPE procedureapp：ds：successively. Sulbactam content was measured by UPLC-MS/MS. The recoveries of sulbactam are 83.3%～116.3%, and the relative standard deviations are 1.9%～8.6%, shows that the method is meet the demand to determine sulbactam content in milk.

关 键 词：牛乳 舒巴坦 UPLC-MS/MS 固相萃取 

分 类 号：TS252.7[轻工技术与工程—农产品加工及贮藏工程]