聚乙烯醇与聚丙烯酸对中温α-淀粉酶的影响  

Effect of poly( vinyl alcohol) and polyacrylic acid on medium temperature α-amylase

作　　者：钱柿汝 袁久刚[1] 姚金龙[2] 樊正国 卢荣清[2] 王强[1] 范雪荣[1] 

机构地区：[1]江南大学生态纺织教育部重点实验室,江苏无锡214122 [2]江苏联发纺织股份有限公司江苏省生态染整技术重点实验室,江苏海安226601

出　　处：《生物学杂志》2014年第5期29-32,共4页Journal of Biology

基　　金：江苏省生态染整技术重点实验室;江苏省纺织上浆工程技术研究中心;江苏省产学研联合创新资金(BY2013015-18)资助项目

摘　　要：研究了聚乙烯醇(PVA)和聚丙烯酸(PAA)对α-淀粉酶活性的影响,并采用荧光光谱法和圆二色谱法分析了PVA和PAA对α-淀粉酶内源性荧光和二级结构的影响。结果表明,PVA和PAA均能使α-淀粉酶的活性降低,并能改变α-淀粉酶的内源性荧光和二级结构,且PVA和PAA的浓度越高,α-淀粉酶活性降低越大,酶的内源性荧光和二级结构的变化也越大。In this paper, the effect of PVA and PAA on the activity of α-amylase was investigated, and the endogenous fluorescence and the secondary structure of α-amylase were also analyzed by using fluorescence spectroscopy and circular dichroism.The results showed that both PVA and PAA could change the endogenous fluorescence and secondary structure ofα-amylase, which resulted in the reduction in the activity ofα-amylase.The more the amount of PVA and PAA added, the more obvious changes were.

关 键 词：Α-淀粉酶 PVA PAA 内源性荧光 二级结构 

分 类 号：TS195.1[轻工技术与工程—纺织化学与染整工程]