五指毛桃组织培养获得再生植株的研究  

Research on tissue culture and plant regeneration for roots of Ficus hirta

作　　者：李林轩[1] 吴庆华[1] 蔡锦源[2] 林伟[1] 韦坤华[1] 

机构地区：[1]广西药用植物园广西药用资源保护与遗传改良重点实验室,广西南宁530023 [2]广西科技大学鹿山学院,广西柳州545616

出　　处：《中草药》2014年第17期2547-2551,共5页Chinese Traditional and Herbal Drugs

基　　金：广西壮族自治区卫生厅中医药科技专项(GZKZ10-081)

摘　　要：目的采用组织培养快繁技术培养五指毛桃种苗,为人工种植提供种源。方法采用五指毛桃叶片作外植体,以MS和1/2 MS为基本培养基,采用正交设计研究植物生长调节剂多因素组合(6-BA、NAA、2,4-D、IAA、KT和IBA)对五指毛桃初代诱导、不定芽分化和诱导生根的影响。结果不定芽最佳诱导培养基为MS+1.0 mg/L 6-BA+0.3 mg/L NAA,外植体经20 d诱导培养,可分化形成不定芽72个;MS+1.0 mg/L 6-BA+0.3 mg/L IAA+0.3 mg/L KT最利于不定芽继代增殖,增殖倍数6.67;1/2 MS+1.0 mg/L IBA+0.3 mg/L NAA最适于诱导生根获得再生植株,生根率100%;宜移栽于泥炭-珍珠岩(1︰1)的基质上,成活率为93%。结论此途径繁殖速度快、再生率高,能为栽培五指毛桃提供大量种苗。Objective To solve the shortage problem of large-scale planting the seedlings in the roots of Ficus hirta by rapid propagation in vitro tissue culture. Methods The MS and 1/2 MS media were used as basic media, The multi factor combination of plant growth regulator（6-BA, NAA, 2, 4-D, IAA, KT, and IBA） on the seedling subculture and root culture was studied by orthogonal design. Results The best medium for the adventitious bud induction was MS ＋ 6-BA 1.0 mg/L ＋ NAA 0.3 mg/L, 72 adventitious buds were obtained by differentiation with 20 d induction of explants; The best medium for cluster inducing and subculture was MS ＋ 1.0 mg/L 6-BA ＋ 0.3 mg/L IAA ＋ 0.3 mg/L KT; The best rooting medium was 1/2 MS ＋ 1.0 mg/L IBA ＋ 0.3 mg/L NAA, and the rooting rate was 100%. Being suitably Transplanted to peat-perlite（1：1） matrix, the 30 d survival rate was 93%. Conclusion The tissue culture for the roots of F. hirta could be used to produce test-tube seedlings for large scale planting.

关 键 词：五指毛桃 组织培养 外植体 快繁技术 植株再生 不定芽 生根 基质 

分 类 号：R282.21[医药卫生—中药学]