底播虾夷扇贝活品供应链各环节中肠道菌群分析  

Intestinal Flora in Supply Chain of Live Released Yesso Scallop Pationopecten yessoensis

作　　者：刘世永[1] 刘俊荣[1] 马永生[1] 沈建[2] 杨婷婷[1] 苗治欧[1] 

机构地区：[1]大连海洋大学,辽宁大连116023 [2]农业部渔业装备与工程重点开放实验室,上海200092

出　　处：《水产科学》2014年第10期626-630,共5页Fisheries Science

基　　金：国家自然科学基金资助项目(31271980);国家现代农业技术产业体系项目(NYCYTX-47)

摘　　要：以鲜活底播虾夷扇贝为研究对象,研究扇贝肠道菌群结构与其生长环境的相似性,并跟踪研究组成供应链的采捕、活水运输、中转、净化Ⅰ、净化Ⅱ及市场等6个环节的活品扇贝的肠道菌群结构变化规律。首先,在扇贝捕捞船现场采集扇贝、海水以及海底沉积物,随后继续对活水运输、中转、净化Ⅰ、净化II及市场等环节跟踪采集扇贝肠道样品;利用基于16SrDNA的PCR-变性梯度凝胶电泳指纹图谱技术对扇贝肠道菌群、沉积物及海水进行分析。变性梯度凝胶电泳指纹图谱分析结明,从即捕扇贝肠道、沉积物及海水中分别获得12、18及20个扩增条带,其中,三者具有9条相同的条带;其余包括活水运输、中转、净化Ⅰ、净化Ⅱ及市场等5个环节的条带数依次为14、16、13、9、22。相似性分析表明,即捕扇贝肠道与海水及沉积物之间菌群组成相似性系数(戴斯系数)分别为66.6%和62.5%;由采捕到市场6环节之间虾夷扇贝肠道菌群相似性系数依次为64.0%、73.3%、69.0%、63.6%及32.3%。采捕后至净化池以及净化后至市场均发生菌群条带的增加,表明采捕后流通环境的改变对扇贝肠道菌群结构有影响;净化处理具有有效的减菌效果;现有捕后处置方式即从采捕至净化Ⅱ,活品扇贝处于比较稳定的胁迫状态,而净化后的产品至消费市场环节的胁迫压力明显,即该零售终端环节对活品质量有重要影响。The similarity of intestinal flora with growing environment and changes in flora structure were studied in yesso scallop Pationopecten yessoensis in live supply chain,including 6key links from post capture on board,live transport,tranship,depurationⅠ,depurationⅡ and marketing.The scallop,seawater and sediments on deck of scallop-dredging vessels were collected,and the scallop was also sampled along the supply chain at each link.The flora in the scallop intestine,seawater and sediment samples was analyzed by 16 SrDNA PCR-DGGE（denaturing gradient gel electrophoresis）fingerprint.The results showed that 12 bands were observed in the intestine,18 bands in the sediments and 20 bands in the seawater,including 9bands being shared.There were 14 bands live transport,16 bands in tranship,13 bands in depurationⅠ,9bands in depurationⅡ and 22bands in markets.Similarity analysis revealed that the similarity was 66.6%between intestinal flora and seawater flora,and 62.5% between the intestinal flora and sediment flora.The similarities were found to be 64%between the intestinal flora and flora in live transport,73.3%between the intestinal flora and flora in tranship,69%between the intestinal flora and flora in depurationⅠ,63.6%between the intestinal flora and flora in depurationⅡ,and 32.3%between the intestinal flora and flora in marketing.It is concluded that DGGE fingerprint band was increased in number from capture to depuration and from post-depuration to market,indicating that the changes in post-capture environment had influence on the flora,and that the depuration leads to reduce in microbiological contamination effectively.The live scallop was in a state of stenoplastic stress from capture to depuration,and in a state of more obvious stress from post-depuration to market,that is,the terminal retail link being a significant impact on the quality of live scallop.

关 键 词：虾夷扇贝 活品流通 肠道菌群结构 

分 类 号：S968.3[农业科学—水产养殖]