鱼藤素对小鼠小胶质细胞BV-2影响的实验研究  

The effects of Deguelin on mouse microglia cell line BV-2

作　　者：熊中奎[1] 郎娟[2] 朱胜裕[1] 王思本[1] 

机构地区：[1]绍兴第二医院放疗科,浙江绍兴312000 [2]绍兴市人民医院医学研究中心,浙江绍兴312000

出　　处：《健康研究》2014年第5期493-495,F0002,共4页Health Research

基　　金：浙江省自然科学基金(Y2100035);浙江省公益性技术应用研究计划(2010C33116);浙江省中医药科技计划(2011ZA108;2012ZB162)

摘　　要：目的研究放射增敏剂鱼藤素对小鼠小胶质细胞BV-2的影响。方法 BV-2细胞以5×10^4/mL密度接种到细胞培养板中,加入8个不同浓度鱼藤素干预48 h后测定细胞增殖活力。在加入1×10^-6mol/L鱼藤素后6个时间点上测定细胞增殖活力。1×10^-8mol/L鱼藤素干预48 h,测定上清液中NO水平。结果 5×10^4/mL BV-2细胞以含10%胎牛血清DMEM培养基培养,第48 h达到细胞生长曲线的峰值。在0-1×10^-5mol/L剂量范围内,1×10^-6、1×10^-5mol/L鱼藤素干预48 h后BV-2细胞的增殖活力显著降低;1×10^-6mol/L鱼藤素加入后第24、48、72h等3个时间点上BV-2细胞增殖活力明显降低。1×10^-8mol/L鱼藤素干预48 h后BV-2细胞上清液NO水平提高。结论鱼藤素在一定的浓度时激活小胶质细胞,更高浓度时将导致小胶质细胞损伤甚至死亡。Objective To understand the effects of deguelin,a radiosensitizer,on mouse microglial cell line BV-2.Method BV-2 cells（5 × 10^4 cells /ml） were seeded in a well in 96-well plate. The cell viability was determined 48 hrs after intervention with Deguelin of eight different density. On the time points of 0,6,12,24,48 or 72 hours after treatment with 1 × 10^-6mol /L deguelin,CCK-8 kits were used to determine cell viability. Nitric oxide levels were measured48 hours after treatment with 1 × 10^-8mol /L deguelin. Findings BV-2 cells reached peak level of growth curve 48 hours after they had been seeded in DMEM medium with 10% fetal bovine serum. Cell viability of BV-2 cells decreased after treatment with deguelin（≥1 × 10^-6mol /L） for 48 hours or after treatment with deguelin（1 × 10^-6mol /L） for more than 24 hours. Supernatant NO level increased after treatment with 1 × 10^-8mol /L deguelin for 48 hours. Conclusion Deguelin of certain concentrations can activate microglial cells. However,Deguelin of higher density can suppress or even kill cell viability.

关 键 词：鱼藤素 放射增敏剂 小胶质细胞 细胞增殖活力 一氧化氮 

分 类 号：R733[医药卫生—肿瘤]