耐高铀浓度氧化亚铁硫杆菌的驯化及其反应动力学  

Domestication and Kinetics Parameters of Thiobacillus Ferrooxidans in High Concentration of Uranium

作　　者：石健[1,2] 王东升[2] 邹开云[1] 

机构地区：[1]南通大学化学化工学院,江苏南通226019 [2]中国科学院生态环境研究中心,北京100085

出　　处：《南通大学学报（自然科学版）》2014年第3期24-27,共4页Journal of Nantong University(Natural Science Edition)　

基　　金：国家自然科学基金项目(21177067);江苏省自然科学基金重点项目(BK2010034);南通市社会发展项目(HS2012001);南通市重点实验室项目(CP12011001);江苏省青蓝工程项目

摘　　要：氧化亚铁硫杆菌在高铀浓度条件下的活性是微生物浸铀技术能否取得成功的关键所在,高浓度铀对细菌有明显的抑制作用,但驯化后可以降低及弱化这种抑制作用.通过考察不同铀浓度对氧化亚铁硫杆菌活性的影响,得出其最佳培养条件并对驯化后菌种对铁的氧化动力学进行了研究,结果表明,该菌在优化条件下培养72 h后,对数期的细菌比生长速率为0.032 2 h-1.The microbial activity of Thiobacillus ferrooxidans in high concentration of uranium is a key to success in microbe leaching technology. Thus, it is significant to deal with the Thiobacillus ferrooxidans and make them adapt to high concentrations of uranium. The influence of the concentration of uranium on the microbial activity of Thiobacillus ferrooxidans was studied in this work, and the optimal culture condition for Thiobacillus ferrooxidans was also achieved. Furthermore, the kinetic parameters of the oxidation of iron by bacteria were researched. The results show that the high content of uranium can restrain the growth of the microbe, however, the restrain process can be reduced by domestication. Nurtured under the optimum culture condition for 72 hours, the growth rate of Thiobacillus ferrooxidans cells is determined to be 0.032 2 h^-1.

关 键 词：氧化亚铁硫杆菌 铀 细菌活性 驯化 动力学 

分 类 号：X172[环境科学与工程—环境科学]