DPPH法评估火棘提取物抗氧化活性体系的建立  

Establishment of Antioxidant Activity System from Pyracantha Fortuneana Fruits by DPPH Evaluation Method

作　　者：曾丹[1] 李旭[1] 高佩[1] 李宁[1] 鄢又玉[1] 

机构地区：[1]武汉轻工大学生物与制药工程学院,武汉430023

出　　处：《中国实验方剂学杂志》2014年第21期79-83,共5页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的：建立DPPH（1,1-二苯基-2-三硝基苯）法火棘提取物抗氧化活性评价体系.方法：主要探讨了DPPH及乙醇浓度、光照、温度及体系pH对DPPH乙醇溶液相对吸光度的影响;以及反应时间与温度、体系pH、光照、稀释样品的乙醇浓度对火棘提取物DPPH抗氧化评价体系的影响.结果：抗氧化评价用DPPH乙醇溶液必须临用新配,浓度应控制在0.02-0.2 mmol·L-1,在冰箱中（2℃）保存时间不能超过4h,且最好避光保存,反应体系适宜的pH为3-8.评价待检样品最好用无水乙醇稀释,加入DPPH于25℃避光反应30 min后,在波长517 nm处测吸光度,通过控制待检样品稀释倍数,使DPPH清除率控制在17.70％ - 53.43％.结论：该实验建立的DPPH法抗氧化活性评价体系,结果稳定可信,重复性好.Objective：To establish evaluation method on antioxidant activity of Pyracantha Fortuneana extract by DPPH.Method：The effect of DPPH and ethanol concentration,light,temperature and pH on relative absorbance of DPPH ethanol solution,as well as the effect of reaction time and temperature,pH,light and ethanol concentration diluting Pyracantha Fortuneana extract were both investigated thoroughly.Result：DPPH ethanol solution must be prepared just before using and can be saved in the refrigerator （2 ℃） for no more than 4 hours without light,whose concentration should be controlled between 0.02 and 0.2 mmol ·L-1 The absorbance of sample solution could be measured at 517nm after being diluted with anhydrous ethanol and reacted for 30 min under 25 ℃ excluding light,along with the optimal pH is from 3 to 8.We had better controlled the sample dilution times and ensured that DPPH clearance ratio is between 17.70％ and 53.43％.Conclusion：This evaluation method is reliable and stable,as well as good repeatability.

关 键 词：1 1-二苯基-2-三硝基苯法 火棘提取物 抗氧化活性 

分 类 号：R284.1[医药卫生—中药学]