橘红多糖增强小鼠免疫功能及抗疲劳作用  

Anti Fatigue Effect and Enhancing Immune by Exocarpium citri Polysaccharide Function

作　　者：王可[1] 尤良震[1] 胡静波 李悌聪 

机构地区：[1]安徽中医药大学,合肥230038 [2]上海同昌生物医药科技有限公司,上海201512

出　　处：《中国实验方剂学杂志》2014年第21期180-182,共3页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:观察橘红多糖对小鼠免疫功能的影响及抗疲劳作用。方法:小鼠分为空白组、模型组、阳性药对照组(10g·kg-1剂量ig贞芪扶正颗粒混悬液)、橘红多糖低、中、高剂量(23,46,92 mg·kg-1),各组每日按10 mL·kg-1ig给药1次,空白和模型组ig给予等体积蒸馏水,持续30 d。第26日,除空白组外以不含2,4-二硝基氟苯(DNFB)的溶剂处理,其余各组以DNFB致敏,耳廓激发的方法建立迟发型超敏反应模型,计算激发后各组小鼠左右耳廓增重值;于第3周后除空白组外给予免疫抑制剂(氢化可的松40 mg·kg-1,肌注,隔天1次),共5次,建立免疫功能低下小鼠模型。计算末次给药1 h后小鼠胸腺、脾脏指数,观测橘红多糖对小鼠免疫功能的影响。采用小鼠负重游泳及耐缺氧试验,观察抗疲劳作用。结果:采用5%DNFB溶液致敏,1%DNFB诱发,制成迟发型超敏反应模型,造模后,小鼠耳显著肿胀,贞芪扶正颗粒组、橘红多糖46,92 mg·kg-1组能够增强迟发超敏效应(P<0.01,P<0.05),小鼠耳肿胀度分别达到(6.57±1.67),(8.29±2.41)mg。采用免疫抑制剂氢化可的松造免疫抑制小鼠模型,造模后小鼠胸腺指数、脾指数下降,贞芪扶正颗粒组、橘红多糖92 mg·kg-1组能显著提高免疫抑制小鼠脾脏及胸腺指数(P<0.01,P<0.05)。贞芪扶正颗粒组、橘红多糖(23,46,92 mg·kg-1)组能够显著延长小鼠负重游泳时间,游泳时间分别为(286±46),(188±34),(227±28),(291±48)s。贞芪扶正颗粒组、橘红多糖3个剂量组均能够显著延长小鼠耐缺氧时间(P<0.01,P<0.05),橘红多糖对增强小鼠免疫和抗疲劳作用均呈现剂量依赖性趋势。结论:橘红多糖具有增强免疫、抗疲劳的作用,多糖是橘红抗炎、增强免疫的有效部位。Objective：To observe the immune promotion and anti-fatigue of effect of Exocarpium citri polysaccharide in mice.Method：KM mice were randomly divided into control group,model group,Zhenqi Fuzheng granule group （10 g ·kg-1）,E.citri polysaccharide low dose group （23 mg ·kg-1）,middle dose group （46 mg ·kg-1）,high dose group （92 mg ·kg-1）.The groups were gavaged （ig） with drug daily for 30 consecutive days,at dose of 10 mL ·kg-1 On 26th day,the delayed-type hypersensitivity animal model in mice was established by sensation of 2,4-dinitro fluoro benzene （DNFB）,the control group was treated with the solvent without DNFB,the weight of right and left ears was measured at 24 h after challenged.Since the third week,dexamethasone was injected to all the groups except for control group every two days to establish immunosuppressed mice model,40 mg ·kg-1 dose,five times.Observed spleen index,thymus index at 1 h after last objection.Using swimming and anti hypoxia experiment to observe the anti fatigue effect.Result：The effect of Zhenqi Fuzheng granules group,the polysaccharide groups at 46,92 mg·kg-1 could enhance the delayed type hypersensitivity （P ＜0.01,P ＜0.05）,the difference of mouse ear swelling was （6.57 ± 1.67）,（8.29 ±2.41） mg,respectively.Zhenqi Fuzheng granule group,polysaccharide group at 92 mg ·kg-1 could significantly increase the immune suppression of thymus and spleen index in mice （P ＜ 0.01,P ＜ 0.05）.Zhenqi Fuzheng granules group,polysaccharide groups at 23,46,92 mg ·kg 1 could significantly prolong the swimming time,swimming time was （286 ±46）,（188 ± 34）,（227 ±28）,（291 ±48） s.Zhenqi Fuzheng granule group,polysaccharide groups at 23,46,92 mg ·kg-1 could significantly prolong the hypoxia tolerance time of mice （P ＜0.01,P ＜0.05）.The effect of enhancement of immune function and anti fatigue action showed a manner of dose depent.Conclusion：E.citri polysaccharide can enhance immunity,and have anti-fatigue effect.Polysaccha

关 键 词：橘红 多糖 增强免疫 抗疲劳 

分 类 号：R285.5[医药卫生—中药学]