青翘正丁醇部位连翘苷、连翘酯苷A的制备及抑菌、抗氧化活性  

Preparation and Antibacterial Antioxidant Activity of Phillyrin,Forsythiaside A on n-butanol Part Extract from Qingqiao of Forsythia suspensa

作　　者：张颖娟[1] 韩燕霞[1] 梁利鹏[1] 尚彩玲[1] 薛慧清[1] 

机构地区：[1]山西中医学院,太原030024

出　　处：《中国实验方剂学杂志》2014年第21期192-196,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：山西省科技攻关项目(20080311052-1)

摘　　要：目的:从青翘正丁醇部位制备连翘苷及连翘酯苷A单体化合物,并对其抑菌活性及清除DPPH自由基的抗氧化能力进行研究。方法:采用硅胶柱层析、重结晶等技术对青翘正丁醇部位连翘苷及连翘酯苷A进行分离纯化;采用K-B纸片扩散法测定连翘苷、连翘酯苷A对大肠埃希菌、金黄色葡萄球菌、肺炎链球菌的抑菌效果;DPPH法测定连翘苷、连翘酯苷A清除DPPH自由基的抗氧化能力。结果:连翘酯苷A对大肠埃希菌、肺炎链球菌及金黄色葡萄球菌抑菌圈的直径分别为12.35,9.58,12.29 mm,连翘酯苷A的抑菌活性高于青翘正丁醇部位总浸膏的抑菌活性,低于庆大霉素的抑菌活性,而连翘苷无抑菌活性,连翘酯苷A对3种细菌的抑制强弱为:大肠埃希菌﹥金黄色葡萄球菌﹥肺炎链球菌;连翘酯苷A清除DPPH自由基的能力大于连翘苷。结论:连翘酯苷A可能是青翘抑菌活性及抗氧化活性的主要成分。Objective：To preparate the phillyrin and forsythiaside A from the n-butanol fraction of Qingqiao of Forsythia suspensa,and to explore their antibacterial activity and free DPPH radical scavenging capacity.Method：The phillyrin and forsythiaside A from the n-butanol fraction of Qingqiao were isolated and purified by means of silica gel column chromatography and recrystallization,etc.The antibacterial activity of phillyrin and forsythiaside A were tested against Escherichia coli,Staphylococcus aureus and Streptococcus pneumoniae by using K-B paper disk method.DPPH assay was employed to assess the antioxidant activity of the phillyrin and forsythiaside A.Result：The diameter of inhibition zone forming by forsythiaside A against E.coli,S.pneumoniae and S.aureus were 12.35,9.58,12.29 mm,respectively.In vitro antibacterial tests revealed that forsythiaside A exhibited better effect than n-butanol fraction of Qingqiao,but not good as gentamicin,and phillyrin didn＇t show any activity.Meanwhile,forsythiaside A was more potent against E.coli than other two strains.Morever,DPPH experimental results showed that forsythiaside A had higher free DPPH radical scavenging capacity than the phillyrin.Conclusion：Forsythiaside A showed a higher antibacterial activity and antioxidant activity in vitro than phillyrin,and it may be the main active components of Qingqiao.

关 键 词：连翘苷 连翘酯苷A 抑菌活性 清除DPPH自由基的抗氧化能力 

分 类 号：R285.5[医药卫生—中药学]