乳杆菌A-2代谢产物对人舌鳞癌CAL-27细胞增殖与凋亡的初步研究  

A preliminary study of lactobacillus sp A-2 metabolites in human tongue squamous carcinoma CAL-27 cell proliferation and apoptosis

作　　者：张国梁[1] 吴江[1] 关键[1] 关宏[2] 李德超[1] 王心彧[1] 

机构地区：[1]佳木斯大学口腔医学院,黑龙江佳木斯154002 [2]齐齐哈尔医学院,黑龙江齐齐哈尔161006

出　　处：《黑龙江医药科学》2014年第5期7-10,共4页Heilongjiang Medicine and Pharmacy

基　　金：佳木斯大学科学技术面上项目;编号:L2012-034

摘　　要：目的:初步研究乳杆菌A-2代谢产物(LSPM1和LSPM2)对人舌鳞癌CAL-27细胞的增殖抑制及诱导细胞凋亡。方法:乳杆菌A-2通过降解制备相应的代谢产物LSPM1和LSPM2;采用MTT法检测不同浓度的LSPM1和LSPM2(1.875mg/mL、3.75mg/mL、7.5mg/mL、15mg/mL和30mg/mL)对CAL-27细胞的体外增殖抑制作用;荧光显微镜下观察CAL-27细胞形态变化;采用流式细胞仪和DNA琼脂凝胶电泳法检测细胞凋亡情况。结果:不同浓度的乳杆菌A-2代谢产物LSPM1和LSPM2可以抑制CAL-27细胞的增殖,呈现明显的浓度依赖性;LPSM1可明显诱导CAL-27细胞凋亡,呈时间依赖性。结论:乳杆菌A-2代谢产物可以在体外抑制人舌鳞癌CAL-27细胞生长增殖,并诱导CAL-27细胞凋亡。Objective. To preliminarily study how lactobacillus sp A--2 metabolites （LSPM1 and LSPM2） restrain human tongue squamous carcinoma CAL--27 cells proliferation and induce its apoptosis. Methods. LSPM1 and LSPM2 were prepared by degradation lactobaeillus sp A--2. It was used to detect different concentrations of LSPM1 and LSPM2 （1. 875mg/mL, 3.75mg/mL, 7.5mg/mL, 15mg/mL, and 30mg/mL） of CAL--27 cells in vitro proliferation inhibition by MTT method. CAL--27 ceil morphological changes were observed by using fluorescence microscope. It was to detect the apoptosis situation of CAL--27 cells by flow cytometry method （FCM） and agarose gel electrophoresis. Results. Different concentrations of LSPM1 and LSPM2 could restrain the growth of CAL--27 cells and had a dose--dependent manner. The apoptosis of CAL --27 cells were obviously induced in time--dependence. Conclusion. Proliferation of CAL--27 cells is inhibited by Lactobaeillus sp A--2 metabolites. Lactobacillus sp A--2 metabolites can induce CAL--27 cells apoptosis.

关 键 词：乳杆菌A-2 代谢产物 人舌鳞癌细胞 细胞凋亡 

分 类 号：R739.86[医药卫生—肿瘤]