蓝光诱导下蛹虫草胞内多糖的单糖组成及虫草素和虫草多糖含量分析  

Study on Monosaccharide Composition of Intracellular Polysacchride and Contents of Cordycepin and Cordyceps Polysacchride Produced by Cordyceps militaris Induced by Blue Light

作　　者：王菊凤[1,2] 杨道德[1] 李鹄鸣[2] 韩文军[3] 

机构地区：[1]中南林业科技大学野生动植物保护研究所,湖南长沙410004 [2]吉首大学生物资源与环境科学学院,湖南吉首416000 [3]中南林业科技大学生命科学与技术学院,湖南长沙410004

出　　处：《中药材》2014年第8期1395-1399,共5页Journal of Chinese Medicinal Materials

基　　金：国家科技部中小企业技术创新基金资助项目(11C26214302946);国家人事部资助项目(国人部发[2004]99号);湖南省重点学科建设资助(CSUFT-201301)

摘　　要：目的:探讨蓝光诱导对蛹虫草Cordyceps militaris L.菌丝体和子实体中胞内多糖的单糖组成以及虫草素和虫草多糖含量的影响。方法:以蛹虫草为材料,使用气相色谱检测蓝光照射144 h后菌丝体及子实体中多糖的单糖组成;并在蓝光照射下的不同时间段分别取样,以检测菌丝体及子实体中虫草素和虫草多糖的含量。同时观察蛹虫草菌丝体和子实体在蓝光照射诱导过程中的生长情况。结果:蛹虫草菌丝体多糖是一种含有4种单糖的杂多糖;子实体多糖是一种含有5种单糖的杂多糖。在菌丝体多糖的检测试验中,无论是蓝光照射还是黑暗培养,两组的多糖含量变化规律几乎相似,都是在一定的时间段开始发生变化。在子实体多糖的检测中,无论是蓝光照射还是白光对照,两组多糖含量的变化规律也基本相同。在菌丝体以及子实体虫草素的检测中,虫草素含量在两组实验中均呈现出明显的上升趋势。结论:蓝光诱导对蛹虫草胞内多糖中单糖的组成种类和数量有一定的影响。而且蓝光诱导对蛹虫草中虫草素和虫草多糖含量的提高有一定的促进作用。蓝光有利于蛹虫草的形态建成,促进子实体的分化和生长发育。该项研究首次报道了蓝光诱导对蛹虫草多糖中单糖组成的种类和数量的影响,为进一步研究蓝光诱导对蛹虫草活性物质的代谢奠定基础。Objective：To study effects of blue light irradiation on monosaccharide composition of intracellular polysacchride and contents of cordycepin and cordyceps polysacchride of mycelium and sporocarp in Cordyceps militaris. Methods：The monosaccharide composition of intracellular polysacchride of mycelium and sporocarp in Cordyceps militaris as materials were determined by gas chromatography after 144 h blue light irradiation. The contents of cordycepin and cordyceps polysacchride of mycelium and sporocarp in Cordyceps militaris were detected at different blue light irradiation periods. At the same time,the growth of mycelium and sporocarp in Cordyceps militaris were observed during blue light irradiation. Results：Mycelium polysaccharide in Cordyceps militaris was a kind of heteropolysaccharide containing four kinds of monosaccharide and fruiting body polysaccharide was a kind of heteropolysaccharide containing five kinds of monosaccharide. Whether blue light irradiation or dark culture,the content changes of cordyceps polysacchride in two groups showed similar patterns in the test of mycelium polysaccharides. The content changes of cordyceps polysacchride in two groups were basically the same in the detection of sporocarp polysacchride. Cordycepin content in the two set of experiments of blue light irradiation all showed a clear upward trend in the detection of mycelium and sporocarp in Cordyceps militaris. Conclusion：The blue light irradiation has certain effect on the species and quantity of monosaccharide in intracellular polysaccharide. The content increase of cordycepin and cordyceps polysacchride in Cordyceps militaris are promoted by blue light irradiation. Blue light can help the morphogenesis and promote the differentiation and growth of sporocarp in Cordyceps militaris. This study is the first report about the effect of blue light on the type and quantity of the monosaccharide composition in polysaccharide of Cordyceps militaris,which will lay the foundation for further study on the metabolism of act

关 键 词：蛹虫草 蓝光诱导 单糖组成 虫草素 虫草多糖 

分 类 号：Q939.9[生物学—微生物学]